Klapperich Lab:Notebook/Lab Meeting Notes/2009/02/26: Difference between revisions

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==26 February 2009 Lab Meeting Agenda==


* Status: compressed air and vacuum. Happening NOW.
Bioanalyzer - 2nd training March 3 9:30am <br>
Pressure sensor - quoted $325 SP70A or $1000+ SP70D, returning old one for them to look <br>
A roll of Kapton film available <br>
† Announcements<br>
*MicroTAS abstracts. <br>
<br>
† Flu R01 <br>
* Up to 130 total. <br>
* 3:30 pm phone conf. on Monday 3/2, Patty and Jim <br>
* Plaque Assay. pfu's 10^4 - 10^6 diretly from samples<br>
* 3/13 Cathie meeting with Nira Pollcak - more samples <br>
* BUMC _ John Connor - Cathie IBC amendment. Two transportation steps <br>
<br>
† SEPSIS Project <br>
* Submitted to Integrative Biology. <br>
*  <br>
<br>
†RNA project
* Will run fresh extract from Anirban with Agilent guy on TU.<br>
* New proposal with Fraunhofer for food.<br>
<br>
† COBRA <br>
* Integration test from here forward. <br>
* Test ongoing with Rubber adhesive tape for Sol-gel. <br>
* Test with engineered SERS substrates <br>
- Still in discussion for dicing <br>
- Same method with Sol-gel <br>
* Flu Concentration and Cleaning. Kit, UCentrifuge, Quote from Beckmann. <br>
** benchtop or floor models searched, price range 32K+, waiting on quote. <br>
<br>
* Substrates (JZ)<br>
- Try glass fixture. <br>
- magnets hold Teflon substrate in place and make sure they sink <br>
- they crack as soon as left dry <br>
- nanopatterned COP provides access to repeatable morphology  *try EBEAM gold on COP <br>
- Engineered COP substrate? <br>
** three categories of making new sbustrates 1) ebeam COP film, 2) COP hot eboss EVAP, 3)stamping/other intermediate method. (JD) <br>
<br>
* Colloid experiment.<br>
- Do Rhodamine. <br>
** ordered <br>
-Need a size specific positive control. PS beads? charged? <br>
** 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate <br>
** co-evaporating diluted PS beads didn't give a signal: difficulty in locating concentrated particles <br>
<br>
† Valve Array/Valve building - done. <br>
<br>
† Fraunhofer: LOAC <br>
* Paper drafting!<br>
<br>
† Biointerfaces group <br>
* <br>
* Final experiments were done. <br>
* counting the number of trapped cells <br>
* adding experimental results in the draft paper.<br>
<br>
† CIMIT- Colson Grant<br>
* IRB still in revision.<br>
*<br>
<br>
† PCR <br>
* Developed a new protocal for PCR coating <br>
* Simulaons of DNA chain model are running (60 beads).  <br>
<br>
† RCA<br>
* Cathie submitted One pager Whitepaper. Waiting for response. <br>
<br>
† HDA<br>
* PReliminary no-valve design for 25 microliters. <br>
* Sonali try to do 10 microliters in the tube <br>
<br>
† Senior project <br>
* Megan<br>
* FEP Teflon film arrived. <br>
* Cutter plotter prototype to be soon bonded. <br>
* Now has access to 3" wafers from MCK. <br>
<br>
† F31: Cochlea <br>
<br>
* Cell/matrix paper blocking controls.  Likely final questions... when is Decorin up/down regulated--- is there an association between fibronectin and actin cytoskeletons during regeneration?  Obtained feedback on culture paper.  <br>
<br>
† Silica Optimization (Lambda): <br>
* Do full spectra on dirty controls - with PG and Without PG <br>
* try another color DNA dye.<br>
*<b>UPDATE The invitrogen Nucleic Acid Stains Dimer Sampler Kit was ordered on Tuesday </b>
http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html
*<b> reading about all the different stains to </b>
Specifically buy
http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail


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Revision as of 15:04, 26 February 2009

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26 February 2009 Lab Meeting Agenda

  • Status: compressed air and vacuum. Happening NOW.

Bioanalyzer - 2nd training March 3 9:30am
Pressure sensor - quoted $325 SP70A or $1000+ SP70D, returning old one for them to look
A roll of Kapton film available

† Announcements

  • MicroTAS abstracts.


† Flu R01

  • Up to 130 total.
  • 3:30 pm phone conf. on Monday 3/2, Patty and Jim
  • Plaque Assay. pfu's 10^4 - 10^6 diretly from samples
  • 3/13 Cathie meeting with Nira Pollcak - more samples
  • BUMC _ John Connor - Cathie IBC amendment. Two transportation steps


† SEPSIS Project

  • Submitted to Integrative Biology.


†RNA project

  • Will run fresh extract from Anirban with Agilent guy on TU.
  • New proposal with Fraunhofer for food.


† COBRA

  • Integration test from here forward.
  • Test ongoing with Rubber adhesive tape for Sol-gel.
  • Test with engineered SERS substrates

- Still in discussion for dicing
- Same method with Sol-gel

  • Flu Concentration and Cleaning. Kit, UCentrifuge, Quote from Beckmann.
    • benchtop or floor models searched, price range 32K+, waiting on quote.


  • Substrates (JZ)

- Try glass fixture.
- magnets hold Teflon substrate in place and make sure they sink
- they crack as soon as left dry
- nanopatterned COP provides access to repeatable morphology *try EBEAM gold on COP
- Engineered COP substrate?

    • three categories of making new sbustrates 1) ebeam COP film, 2) COP hot eboss EVAP, 3)stamping/other intermediate method. (JD)


  • Colloid experiment.

- Do Rhodamine.

    • ordered

-Need a size specific positive control. PS beads? charged?

    • 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate
    • co-evaporating diluted PS beads didn't give a signal: difficulty in locating concentrated particles


† Valve Array/Valve building - done.


† Fraunhofer: LOAC

  • Paper drafting!


† Biointerfaces group


  • Final experiments were done.
  • counting the number of trapped cells
  • adding experimental results in the draft paper.


† CIMIT- Colson Grant

  • IRB still in revision.


† PCR

  • Developed a new protocal for PCR coating
  • Simulaons of DNA chain model are running (60 beads).


† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.


† HDA

  • PReliminary no-valve design for 25 microliters.
  • Sonali try to do 10 microliters in the tube


† Senior project

  • Megan
  • FEP Teflon film arrived.
  • Cutter plotter prototype to be soon bonded.
  • Now has access to 3" wafers from MCK.


† F31: Cochlea

  • Cell/matrix paper blocking controls. Likely final questions... when is Decorin up/down regulated--- is there an association between fibronectin and actin cytoskeletons during regeneration? Obtained feedback on culture paper.


† Silica Optimization (Lambda):

  • Do full spectra on dirty controls - with PG and Without PG
  • try another color DNA dye.
  • UPDATE The invitrogen Nucleic Acid Stains Dimer Sampler Kit was ordered on Tuesday

http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html

  • reading about all the different stains to

Specifically buy http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail


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