Klapperich Lab:Notebook/Lab Meeting Notes/2009/02/26: Difference between revisions
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* Status: compressed air and vacuum. Happening NOW. | * Status: compressed air and vacuum. Happening NOW. | ||
Bioanalyzer <br> | Bioanalyzer - 2nd training March 3 9:30am <br> | ||
Pressure sensor <br> | Pressure sensor - quoted $325 SP70A or $1000+ SP70D, returning old one for them to look <br> | ||
A roll of Kapton film available <br> | |||
† Announcements<br> | † Announcements<br> | ||
| Line 17: | Line 18: | ||
<br> | <br> | ||
† Flu R01 <br> | † Flu R01 <br> | ||
* | * Up to 130 total. <br> | ||
* Plaque Assay. <br> | * 3:30 pm phone conf. on Monday 3/2, Patty and Jim <br> | ||
* <br> | * Plaque Assay. pfu's 10^4 - 10^6 diretly from samples<br> | ||
* 3/13 Cathie meeting with Nira Pollcak - more samples <br> | |||
* BUMC _ John Connor - Cathie IBC amendment. Two transportation steps <br> | |||
<br> | <br> | ||
† SEPSIS Project <br> | † SEPSIS Project <br> | ||
* Submitted to Integrative Biology. <br> | * Submitted to Integrative Biology. <br> | ||
* <br> | |||
* <br> | |||
<br> | <br> | ||
†RNA project | †RNA project | ||
* | * Will run fresh extract from Anirban with Agilent guy on TU.<br> | ||
* | * New proposal with Fraunhofer for food.<br> | ||
<br> | <br> | ||
† COBRA | † COBRA <br> | ||
* Integration test from here forward. <br> | * Integration test from here forward. <br> | ||
- | * Test ongoing with Rubber adhesive tape for Sol-gel. <br> | ||
* Test with engineered SERS substrates <br> | * Test with engineered SERS substrates <br> | ||
- Still in discussion for dicing <br> | |||
- Same method with Sol-gel <br> | |||
* Flu Concentration and Cleaning. Kit, UCentrifuge, Quote from Beckmann. <br> | * Flu Concentration and Cleaning. Kit, UCentrifuge, Quote from Beckmann. <br> | ||
** benchtop or floor models searched, price range 32K+, waiting on quote. <br> | ** benchtop or floor models searched, price range 32K+, waiting on quote. <br> | ||
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- magnets hold Teflon substrate in place and make sure they sink <br> | - magnets hold Teflon substrate in place and make sure they sink <br> | ||
- they crack as soon as left dry <br> | - they crack as soon as left dry <br> | ||
- nanopatterned COP provides access to repeatable morphology <br> | - nanopatterned COP provides access to repeatable morphology *try EBEAM gold on COP <br> | ||
- <br> | - Engineered COP substrate? <br> | ||
* Colloid experiment. | ** three categories of making new sbustrates 1) ebeam COP film, 2) COP hot eboss EVAP, 3)stamping/other intermediate method. (JD) <br> | ||
<br> | |||
* Colloid experiment.<br> | |||
- Do Rhodamine. <br> | - Do Rhodamine. <br> | ||
** ordered <br> | |||
-Need a size specific positive control. PS beads? charged? <br> | -Need a size specific positive control. PS beads? charged? <br> | ||
* 1um PS | ** 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate <br> | ||
* co-evaporating didn't give a signal: difficulty in locating concentrated particles <br> | ** co-evaporating diluted PS beads didn't give a signal: difficulty in locating concentrated particles <br> | ||
<br> | <br> | ||
† Valve Array/Valve building. <br> | † Valve Array/Valve building - done. <br> | ||
<br> | <br> | ||
† Fraunhofer: LOAC <br> | † Fraunhofer: LOAC <br> | ||
| Line 76: | Line 80: | ||
<br> | <br> | ||
† HDA<br> | † HDA<br> | ||
* | * PReliminary no-valve design for 25 microliters. <br> | ||
* | * Sonali try to do 10 microliters in the tube <br> | ||
<br> | <br> | ||
† Senior project <br> | † Senior project <br> | ||
* Megan<br> | * Megan<br> | ||
* Cutter plotter | * FEP Teflon film arrived. <br> | ||
* | * Cutter plotter prototype to be soon bonded. <br> | ||
* Now has access to 3" wafers from MCK. <br> | |||
<br> | <br> | ||
† F31: Cochlea <br> | † F31: Cochlea <br> | ||
| Line 90: | Line 95: | ||
† Silica Optimization (Lambda): <br> | † Silica Optimization (Lambda): <br> | ||
* | * Do full spectra on dirty controls - with PG and Without PG <br> | ||
* try another color DNA dye.<br> | |||
* try another color DNA dye. | |||
*<b>UPDATE The invitrogen Nucleic Acid Stains Dimer Sampler Kit was ordered on Tuesday </b> | *<b>UPDATE The invitrogen Nucleic Acid Stains Dimer Sampler Kit was ordered on Tuesday </b> | ||
http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html | http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html | ||
*<b> reading about all the different stains to </b> | |||
Specifically buy | Specifically buy | ||
Revision as of 15:04, 26 February 2009
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26 February 2009 Lab Meeting Agenda
Bioanalyzer - 2nd training March 3 9:30am † Announcements
- Still in discussion for dicing
- Try glass fixture.
- Do Rhodamine.
-Need a size specific positive control. PS beads? charged?
† Silica Optimization (Lambda):
Specifically buy http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail | |
