Klapperich Lab:Notebook/Lab Meeting Notes/2009/02/26: Difference between revisions

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==26 February 2009 Lab Meeting Agenda==


* Status: compressed air and vacuum. Happening NOW.
† Announcements<br>
*MicroTAS abstracts. <br>
<br>
† Flu R01 <br>
* Modified Schedule this week. <br>
* Plaque Assay. <br>
* <br>
<br>
† SEPSIS Project <br>
* Submitted to Integrative Biology. <br>
*  <br>.
*  <br>
<br>
†RNA project
* Samples Sent? <br>
*  New proposal with Fraunhofer for food. <br>
<br>
† COBRA <br>
* Mark- Control circuit - done, need to be tested.<br>
* Integration test from here forward. <br>
- Direct measurement with engineered SERS substrate.<br>
* Test with engineered SERS substrates <br>
* Flu Concentration and Cleaning. Kit, UCentrifuge, Quote from Beckmann. <br>
<br>
* Substrates (JZ)<br>
- Try glass fixture. <br>
- <br>
- <br>
* Colloid experiment.  <br>
- Do Rhodamine. <br>
-Need a size specific positive control. PS beads? charged? <br>
*<br>
<br>
† Valve Array/Valve building. <br>
*  Parts missing. Check with Susan.<br>
* Writing a new control interface. <br>
<br>
† Fraunhofer: LOAC <br>
* Paper drafting!<br>
<br>
† Biointerfaces group <br>
* <br>
* Final experiments were done. <br>
* counting the number of trapped cells <br>
* adding experimental results in the draft paper.<br>
<br>
† CIMIT- Colson Grant<br>
* IRB still in revision.<br>
*<br>
<br>
† PCR <br>
* surface coating of zeonex, looking for an effective way to verify the PEG layer <br>
* tried contact angle, device is still broken<br>
* SEM showed no pattern on the plain zeonex without plasma treatment<br>
* XPS?  <br>
* detect roughness with zygo<br>
* Simulaons of DNA chain model are running (60 beads).  <br>
<br>
† RCA<br>
* Cathie submitted One pager Whitepaper. Waiting for response. <br>
<br>
† HDA<br>
* Large volume filling and valve tested. <br>
* Design issue - whole integration or assemble parts (SPE & HDA chamber) <br>
<br>
† Senior project <br>
* Megan<br>
* Cutter plotter for fast prototype.<br>
* Need to get back in cleanroom. Hussam?  Get 3" wafers from MCK. <br>
<br>
† F31: Cochlea <br>
<br>
* Nathan out this week.<br>
<br>
† Silica Optimization (Lambda): <br>
* Overview to look at the data collectively [http://openwetware.org/wiki/Image:Overview.xls] <br>
* Next E. coli. <br>
* try another color DNA dye.
*<b>UPDATE The invitrogen Nucleic Acid Stains Dimer Sampler Kit was ordered on Tuesday </b>
http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html
Specifically buy
http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail


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26 February 2009 Lab Meeting Agenda

  • Status: compressed air and vacuum. Happening NOW.

† Announcements

  • MicroTAS abstracts.


† Flu R01

  • Modified Schedule this week.
  • Plaque Assay.


† SEPSIS Project

  • Submitted to Integrative Biology.

  • .


†RNA project

  • Samples Sent?
  • New proposal with Fraunhofer for food.


† COBRA

  • Mark- Control circuit - done, need to be tested.
  • Integration test from here forward.

- Direct measurement with engineered SERS substrate.

  • Test with engineered SERS substrates
  • Flu Concentration and Cleaning. Kit, UCentrifuge, Quote from Beckmann.


  • Substrates (JZ)

- Try glass fixture.
-
-

  • Colloid experiment.

- Do Rhodamine.
-Need a size specific positive control. PS beads? charged?



† Valve Array/Valve building.

  • Parts missing. Check with Susan.
  • Writing a new control interface.


† Fraunhofer: LOAC

  • Paper drafting!


† Biointerfaces group


  • Final experiments were done.
  • counting the number of trapped cells
  • adding experimental results in the draft paper.


† CIMIT- Colson Grant

  • IRB still in revision.


† PCR

  • surface coating of zeonex, looking for an effective way to verify the PEG layer
  • tried contact angle, device is still broken
  • SEM showed no pattern on the plain zeonex without plasma treatment
  • XPS?
  • detect roughness with zygo
  • Simulaons of DNA chain model are running (60 beads).


† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.


† HDA

  • Large volume filling and valve tested.
  • Design issue - whole integration or assemble parts (SPE & HDA chamber)


† Senior project

  • Megan
  • Cutter plotter for fast prototype.
  • Need to get back in cleanroom. Hussam? Get 3" wafers from MCK.


† F31: Cochlea

  • Nathan out this week.


† Silica Optimization (Lambda):

  • Overview to look at the data collectively [1]
  • Next E. coli.
  • try another color DNA dye.
  • UPDATE The invitrogen Nucleic Acid Stains Dimer Sampler Kit was ordered on Tuesday

http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html

Specifically buy http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail


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