26 February 2009 Lab Meeting Agenda
- Status: compressed air and vacuum. Happening NOW.
Bioanalyzer
Pressure sensor
† Announcements
† Flu R01
- Modified Schedule this week.
- Plaque Assay.
† SEPSIS Project
- Submitted to Integrative Biology.
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†RNA project
- Samples Sent?
- New proposal with Fraunhofer for food.
† COBRA
- Mark- Control circuit - done, need to be tested.
- Integration test from here forward.
- Direct measurement with engineered SERS substrate.
- Test with engineered SERS substrates
- Flu Concentration and Cleaning. Kit, UCentrifuge, Quote from Beckmann.
- benchtop or floor models searched, price range 32K+, waiting on quote.
- Try glass fixture.
- magnets hold Teflon substrate in place and make sure they sink
- they crack as soon as left dry
- nanopatterned COP provides access to repeatable morphology
- Engineered COP substrate?
- Do Rhodamine.
-Need a size specific positive control. PS beads? charged?
- 1um PS bead gives distinctive spectrum on Ranjith's substrate
- co-evaporating didn't give a signal: difficulty in locating concentrated particles
† Valve Array/Valve building.
- Parts missing. Check with Susan.
- Writing a new control interface.
† Fraunhofer: LOAC
† Biointerfaces group
- Final experiments were done.
- counting the number of trapped cells
- adding experimental results in the draft paper.
† CIMIT- Colson Grant
† PCR
- Developed a new protocal for PCR coating
- Simulaons of DNA chain model are running (60 beads).
† RCA
- Cathie submitted One pager Whitepaper. Waiting for response.
† HDA
- Large volume filling and valve tested.
- Design issue - whole integration or assemble parts (SPE & HDA chamber)
† Senior project
- Megan
- Cutter plotter for fast prototype.
- Need to get back in cleanroom. Hussam? Get 3" wafers from MCK.
† F31: Cochlea
- Cell/matrix paper blocking controls. Likely final questions... when is Decorin up/down regulated--- is there an association between fibronectin and actin cytoskeletons during regeneration? Obtained feedback on culture paper.
† Silica Optimization (Lambda):
- Overview to look at the data collectively [1]
- try another color DNA dye.
- UPDATE The invitrogen Nucleic Acid Stains Dimer Sampler Kit was ordered on Tuesday
http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html
Specifically buy
http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail
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