View source for Klapperich Lab:Notebook/Lab Meeting Notes/2009/03/19

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==19 March 2009 Lab UPDATE - NO MEETING ==

Pressure sensor - quoted $325 SP70A or $1000+ SP70D, returning old one for them to look <br>
*  Co. says that it works fine. Transducer up to 1000 psi. We are getting it back. <br>
STATUS?

† Announcements<br>
*MicroTAS abstracts. I NEED THESE BY 3/18 so I can edit them!!! <br>
<br>
† Flu R01 <br>
* I need an update on where we are in the RT PCR process with the samples (off-chip). What PCR are we doing? The M1 gene, or something else.
* BUMC _ John Connor - Cathie STILL working on IBC amendment. Two transportation steps <br>
<br>
† SEPSIS Project <br>
* Submitted to LOC. <br>
*  <br> 
<br>
†RNA project
* Need to get Jurkat RNA to Jeff. <br>
* <br>
<br>
† COBRA <br> 
* Integration test from here forward. <br>
* 
<br>
* Substrates (JZ)<br>
- nanopatterned COP provides access to repeatable morphology   <br>
- Engineered COP substrate? UPDATE? <br>
*three categories of making new sbustrates 1) ebeam COP film, 2) COP hot eboss EVAP (Luca), 3)stamping/other intermediate method(Bjoern similar). (JD) <br>
<br>
* Colloid experiment.<br>
- Do Rhodamine. <br>
** ordered <br>
-Need a size specific positive control. PS beads? charged? <br>
** 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate <br>
** co-evaporating diluted PS beads didn't give a signal: difficulty in locating concentrated particles <br>
<br>
† Valve Array/Valve building - done. <br>

<br>
† Fraunhofer: LOAC <br>
* Paper submitted to LOC<br>
<br>
† Biointerfaces group <br>
* Cathie in charge of Bionterfaces Paper1 (trapping?) ? Let me know. <br>
* Jason promised me add his change part soon. <br>
* I will change Fig. 2a-b (10 um channel model) <br>
* making one abstract for MicroTAS based on numerical results of 20 um channel model. <br>
* designing herringbone groove for better trapping <br) 
* investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).<br>
<br>
† CIMIT- Colson Grant<br>
* IRB still in revision.<br>
*<br>
<br>
† PCR <br>
* Cathie in charge of PCR1 paper right now. <br>
* Developed a new protocal for PCR coating <br>
* will make figures for PCR1 paper. <br>
* developing large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles) <br>
* making one abstract for MicroTAS (similar to the abstract of AACC) <br>
<br>
† RCA/HDA<br>
* Cathie submitted One pager Whitepaper. Waiting for response. <br>
* convert this whitepaper to grant? RCA/HDA on a chip for NIH...anyone interested? ANYONE?<br>
* Preliminary no-valve design for 25 microliters. <br>
* 20 more test chips to Sonali (AACC poster). <br>
* Sonali try to do 10 microliters in the tube <br>
<br>
† Senior project <br>
* Megan<br> 
* Test on Thu, Fri worked but failure after some cycles. <br>
* Need to enhance bonding (e.g. Ar or O2 ashing)<br>
<br>
† F31: Cochlea <br>
<br>
* STILL NEED TO SCHEDULE MEETING  <br>
<br>

† Silica Optimization (Lambda): <br>
* New dataset? <br>
* In order to get bind/release info below 2 ng, we will need to do qPCR. Can we use Qingqing's lambda phage assay for this analysis? We will need this data to characterize the flu chips. <br>

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