Klapperich Lab:Notebook/Lab Meeting Notes/2009/03/19: Difference between revisions

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† Flu R01 <br>
† Flu R01 <br>
* I need an update on where we are in the RT PCR process with the samples (off-chip). What PCR are we doing? The M1 gene, or something else.
* I need an update on where we are in the RT PCR process with the samples (off-chip). What PCR are we doing? The M1 gene, or something else.
on-chip PCR is for M1 gene,93bp, same as sonali's assay.
* BUMC _ John Connor - Cathie STILL working on IBC amendment. Two transportation steps <br>
* BUMC _ John Connor - Cathie STILL working on IBC amendment. Two transportation steps <br>
<br>
<br>
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† PCR <br>
† PCR <br>
* Cathie in charge of PCR1 paper right now. <br>
* Cathie in charge of PCR1 paper right now. <br>
* Developed a new protocal for PCR coating <br>
* Qingqing has written the introduction <br>
* tested the PCR efficiency for different designs of channel.<br>
* new design 1 has much better performance than the original one, while new design 2 has worse performance <br>
* evaluation of PCR efficiency from fluorescent signal and molarity is different.
* will make figures for PCR1 paper. <br>
* will make figures for PCR1 paper. <br>
* developing large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles) <br>
* developing large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles) <br>
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† Silica Optimization (Lambda): <br>
† Silica Optimization (Lambda): <br>
* New dataset? Datasets for all the experiments will be reported on Tuesday<br>
*The following will be submitted monday<br>
*I'm doing total DNA of 5, 10 , 20 , 100 and 200 ng. I didn't do below 2 ng, but thats not a big deal<br>
<br>
On Tuesday All report the data for all of these experiments.<br>
*total of 20 channels 10 as negative and 10 positive with 100ng total NA mass. Emission spectrum of negative samples with excitation at 488nm.<br>
*Emission spectrum of negative samples at excitations corresponding to the NA sampler kit dyes<br>
*Emission spectrum for the positive samples split into 2 groups , one with pico green and the other without at 488nm .<br>
*Chip ran with total of 5ng of NA , pico green using the low range standard curve<br>


* In order to get bind/release info below 2 ng, we will need to do qPCR. Can we use Qingqing's lambda phage assay for this analysis? We will need this data to characterize the flu chips.
<br>
*I'll ask Sonali and Qingqing about this<br>




Revision as of 10:33, 26 March 2009

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19 March 2009 Lab UPDATE - NO MEETING

Pressure sensor - quoted $325 SP70A or $1000+ SP70D, returning old one for them to look

  • Co. says that it works fine. Transducer up to 1000 psi. We are getting it back.

STATUS?

† Announcements

  • MicroTAS abstracts. I NEED THESE BY 3/18 so I can edit them!!!


† Flu R01

  • I need an update on where we are in the RT PCR process with the samples (off-chip). What PCR are we doing? The M1 gene, or something else.

on-chip PCR is for M1 gene,93bp, same as sonali's assay.

  • BUMC _ John Connor - Cathie STILL working on IBC amendment. Two transportation steps


† SEPSIS Project

  • Submitted to LOC.


†RNA project

  • Need to get Jurkat RNA to Jeff.


† COBRA

  • Integration test from here forward.


  • Substrates (JZ)

- nanopatterned COP provides access to repeatable morphology
- Engineered COP substrate? UPDATE?

  • three categories of making new sbustrates 1) ebeam COP film, 2) COP hot eboss EVAP (Luca), 3)stamping/other intermediate method(Bjoern similar). (JD)


  • Colloid experiment.

- Do Rhodamine.

    • ordered

-Need a size specific positive control. PS beads? charged?

    • 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate
    • co-evaporating diluted PS beads didn't give a signal: difficulty in locating concentrated particles


† Valve Array/Valve building - done.


† Fraunhofer: LOAC

  • Paper submitted to LOC


† Biointerfaces group

  • Cathie in charge of Bionterfaces Paper1 (trapping?) ? Let me know.
  • Jason promised me add his change part soon.
  • I will change Fig. 2a-b (10 um channel model)
  • making one abstract for MicroTAS based on numerical results of 20 um channel model.
  • designing herringbone groove for better trapping <br)
  • investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).


† CIMIT- Colson Grant

  • IRB still in revision.


† PCR

  • Cathie in charge of PCR1 paper right now.
  • Qingqing has written the introduction
  • tested the PCR efficiency for different designs of channel.
  • new design 1 has much better performance than the original one, while new design 2 has worse performance
  • evaluation of PCR efficiency from fluorescent signal and molarity is different.
  • will make figures for PCR1 paper.
  • developing large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)
  • making one abstract for MicroTAS (similar to the abstract of AACC)


† RCA/HDA

  • Cathie submitted One pager Whitepaper. Waiting for response.
  • convert this whitepaper to grant? RCA/HDA on a chip for NIH...anyone interested? ANYONE?
  • Preliminary no-valve design for 25 microliters.
  • 20 more test chips to Sonali (AACC poster).
  • Sonali try to do 10 microliters in the tube


† Senior project

  • Megan
  • Test on Thu, Fri worked but failure after some cycles.
  • Need to enhance bonding (e.g. Ar or O2 ashing)


† F31: Cochlea

  • STILL NEED TO SCHEDULE MEETING


† Silica Optimization (Lambda):

  • The following will be submitted monday


  • total of 20 channels 10 as negative and 10 positive with 100ng total NA mass. Emission spectrum of negative samples with excitation at 488nm.
  • Emission spectrum of negative samples at excitations corresponding to the NA sampler kit dyes
  • Emission spectrum for the positive samples split into 2 groups , one with pico green and the other without at 488nm .
  • Chip ran with total of 5ng of NA , pico green using the low range standard curve



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