Klapperich Lab:Notebook/Lab Meeting Notes/2009/03/19

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(19 March 2009 Lab UPDATE - NO MEETING)
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† Silica Optimization (Lambda): <br>
† Silica Optimization (Lambda): <br>
-
* New dataset? <br>
+
* New dataset? Datasets for all the experiments will be reported on Tuesday<br>
-
* In order to get bind/release info below 2 ng, we will need to do qPCR. Can we use Qingqing's lambda phage assay for this analysis? We will need this data to characterize the flu chips. <br>
+
*I'm doing total DNA of 5, 10 , 20 , 100 and 200 ng. I didn't do below 2 ng, but thats not a big deal<br>
 +
On Tuesday All report the data for all of these experiments.<br>
 +
 
 +
* In order to get bind/release info below 2 ng, we will need to do qPCR. Can we use Qingqing's lambda phage assay for this analysis? We will need this data to characterize the flu chips.
 +
<br>
 +
*I'll ask Sonali and Qingqing about this<br>
 +
 
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Revision as of 16:52, 15 March 2009

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19 March 2009 Lab UPDATE - NO MEETING

Pressure sensor - quoted $325 SP70A or $1000+ SP70D, returning old one for them to look

  • Co. says that it works fine. Transducer up to 1000 psi. We are getting it back.

STATUS?

† Announcements

  • MicroTAS abstracts. I NEED THESE BY 3/18 so I can edit them!!!


† Flu R01

  • I need an update on where we are in the RT PCR process with the samples (off-chip). What PCR are we doing? The M1 gene, or something else.
  • BUMC _ John Connor - Cathie STILL working on IBC amendment. Two transportation steps


† SEPSIS Project

  • Submitted to LOC.


†RNA project

  • Need to get Jurkat RNA to Jeff.


† COBRA

  • Integration test from here forward.


  • Substrates (JZ)

- nanopatterned COP provides access to repeatable morphology
- Engineered COP substrate? UPDATE?

  • three categories of making new sbustrates 1) ebeam COP film, 2) COP hot eboss EVAP (Luca), 3)stamping/other intermediate method(Bjoern similar). (JD)


  • Colloid experiment.

- Do Rhodamine.

    • ordered

-Need a size specific positive control. PS beads? charged?

    • 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate
    • co-evaporating diluted PS beads didn't give a signal: difficulty in locating concentrated particles


† Valve Array/Valve building - done.


† Fraunhofer: LOAC

  • Paper submitted to LOC


† Biointerfaces group

  • Cathie in charge of Bionterfaces Paper1 (trapping?) ? Let me know.
  • Jason promised me add his change part soon.
  • I will change Fig. 2a-b (10 um channel model)
  • making one abstract for MicroTAS based on numerical results of 20 um channel model.
  • designing herringbone groove for better trapping <br)
  • investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).


† CIMIT- Colson Grant

  • IRB still in revision.


† PCR

  • Cathie in charge of PCR1 paper right now.
  • Developed a new protocal for PCR coating
  • will make figures for PCR1 paper.
  • developing large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)
  • making one abstract for MicroTAS (similar to the abstract of AACC)


† RCA/HDA

  • Cathie submitted One pager Whitepaper. Waiting for response.
  • convert this whitepaper to grant? RCA/HDA on a chip for NIH...anyone interested? ANYONE?
  • Preliminary no-valve design for 25 microliters.
  • 20 more test chips to Sonali (AACC poster).
  • Sonali try to do 10 microliters in the tube


† Senior project

  • Megan
  • Test on Thu, Fri worked but failure after some cycles.
  • Need to enhance bonding (e.g. Ar or O2 ashing)


† F31: Cochlea

  • STILL NEED TO SCHEDULE MEETING


† Silica Optimization (Lambda):

  • New dataset? Datasets for all the experiments will be reported on Tuesday
  • I'm doing total DNA of 5, 10 , 20 , 100 and 200 ng. I didn't do below 2 ng, but thats not a big deal

On Tuesday All report the data for all of these experiments.

  • In order to get bind/release info below 2 ng, we will need to do qPCR. Can we use Qingqing's lambda phage assay for this analysis? We will need this data to characterize the flu chips.


  • I'll ask Sonali and Qingqing about this



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