Klapperich Lab:Notebook/Lab Meeting Notes/2009/03/19

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19 March 2009 Lab UPDATE - NO MEETING

Pressure sensor - quoted $325 SP70A or $1000+ SP70D, returning old one for them to look

  • Co. says that it works fine. Transducer up to 1000 psi. We are getting it back.

STATUS?

† Announcements

  • MicroTAS abstracts. I NEED THESE BY 3/18 so I can edit them!!!


† Flu R01

  • I need an update on where we are in the RT PCR process with the samples (off-chip). What PCR are we doing? The M1 gene, or something else.
  • BUMC _ John Connor - Cathie STILL working on IBC amendment. Two transportation steps


† SEPSIS Project

  • Submitted to LOC.


†RNA project

  • Need to get Jurkat RNA to Jeff.


† COBRA

  • Integration test from here forward.


  • Substrates (JZ)

- nanopatterned COP provides access to repeatable morphology
- Engineered COP substrate? UPDATE?

  • three categories of making new sbustrates 1) ebeam COP film, 2) COP hot eboss EVAP (Luca), 3)stamping/other intermediate method(Bjoern similar). (JD)


  • Colloid experiment.

- Do Rhodamine.

    • ordered

-Need a size specific positive control. PS beads? charged?

    • 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate
    • co-evaporating diluted PS beads didn't give a signal: difficulty in locating concentrated particles


† Valve Array/Valve building - done.


† Fraunhofer: LOAC

  • Paper submitted to LOC


† Biointerfaces group

  • Cathie in charge of Bionterfaces Paper1 (trapping?) ? Let me know.
  • Final experiments were done.
  • counting the number of trapped cells
  • adding experimental results in the draft paper.


† CIMIT- Colson Grant

  • IRB still in revision.


† PCR

  • Cathie in charge of PCR1 paper right now.
  • Developed a new protocal for PCR coating
  • Simulaons of DNA chain model are running (60 beads).


† RCA/HDA

  • Cathie submitted One pager Whitepaper. Waiting for response.
  • convert this whitepaper to grant? RCA/HDA on a chip for NIH...anyone interested? ANYONE?
  • Preliminary no-valve design for 25 microliters.
  • 20 more test chips to Sonali (AACC poster).
  • Sonali try to do 10 microliters in the tube


† Senior project

  • Megan
  • Test on Thu, Fri worked but failure after some cycles.
  • Need to enhance bonding (e.g. Ar or O2 ashing)


† F31: Cochlea

  • STILL NEED TO SCHEDULE MEETING


† Silica Optimization (Lambda):

  • New dataset?
  • In order to get bind/release info below 2 ng, we will need to do qPCR. Can we use Qingqing's lambda phage assay for this analysis? We will need this data to characterize the flu chips.