Klapperich Lab:Notebook/Lab Meeting Notes/2009/04/09: Difference between revisions
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==9 April 2009 Lab Meeting== | |||
Pressure sensor - quoted $325 SP70A or $1000+ SP70D, returning old one for them to look <br> | |||
* Co. says that it works fine. Transducer up to 1000 psi. We are getting it back. <br> | |||
STATUS? | |||
† Announcements<br> | |||
*Need posters for Oakridge soon. <br> | |||
<br> | |||
† Flu R01 <br> | |||
* | |||
* <br> | |||
<br> | |||
† SEPSIS Project <br> | |||
* Submitted to LOC. <br> | |||
* <br> | |||
<br> | |||
†RNA project | |||
* Need to get Jurkat RNA to Jeff. He may visit in May. <br> | |||
* <br> | |||
<br> | |||
† COBRA <br> | |||
* Integration update? Let's get some of this done before JD leaves for Korea. <br> | |||
* | |||
<br> | |||
* Substrates (JZ)<br> | |||
** Let's get this to a point where we can publish and move on....JZ and CMK to discuss. | |||
- nanopatterned COP provides access to repeatable morphology <br> | |||
- Engineered COP substrate? UPDATE? <br> | |||
*three categories of making new sbustrates 1) ebeam COP film, 2) COP hot eboss EVAP (Luca), 3)stamping/other intermediate method(Bjoern similar). (JD) <br> | |||
<br> | |||
* Colloid experiment.<br> | |||
- Do Rhodamine. <br> | |||
** ordered <br> | |||
-Need a size specific positive control. PS beads? charged? <br> | |||
** 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate <br> | |||
** co-evaporating diluted PS beads didn't give a signal: difficulty in locating concentrated particles <br> | |||
<br> | |||
† Valve Array/Valve building - done. <br> | |||
<br> | |||
† Fraunhofer: LOAC <br> | |||
* Paper submitted to LOC<br> | |||
<br> | |||
† Biointerfaces group <br> | |||
* Cathie in charge of Bionterfaces Paper1. Cathie has final draft now before it goes to collaborators. <br> | |||
* | |||
* designing herringbone groove for better trapping <br> | |||
* investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).<br> | |||
<br> | |||
† CIMIT- Colson Grant<br> | |||
* IRB still in revision.<br> | |||
*<br> | |||
<br> | |||
† PCR <br> | |||
* Cathie read and gave back PCR 1 paper to QQ and MCK<br> | |||
* Qingqing has written the introduction <br> | |||
* repeated test of the PCR efficiency for different designs of channel.<br> | |||
* test PCR efficiency using smaller DNA size (63bp product) | |||
* analyze PCR product for different temperature using bioanalyzer. | |||
* will make figures for PCR1 paper. <br> | |||
* developing large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles) <br> <br> | |||
<br> | |||
† RCA/HDA<br> | |||
* Cathie submitted One pager Whitepaper. Waiting for response. <br> | |||
* convert this whitepaper to grant? RCA/HDA on a chip for NIH...anyone interested? ANYONE?<br> | |||
* Preliminary no-valve design for 25 microliters. <br> | |||
* 20 more test chips to Sonali (AACC poster). <br> | |||
* Sonali try to do 10 microliters in the tube <br> | |||
<br> | |||
† Senior project <br> | |||
* Megan<br> | |||
* Test on Thu, Fri worked but failure after some cycles. <br> | |||
* Need to enhance bonding (e.g. Ar or O2 ashing)<br> | |||
<br> | |||
† F31: Cochlea <br> | |||
<br> | |||
* <br> | |||
<br> | |||
† Silica Optimization (Lambda): <br> | |||
*The following will be submitted monday<br> | |||
<br> | |||
*total of 20 channels 10 as negative and 10 positive with 100ng total NA mass. Emission spectrum of negative samples with excitation at 488nm.<br> | |||
*Emission spectrum of negative samples at excitations corresponding to the NA sampler kit dyes<br> | |||
*Emission spectrum for the positive samples split into 2 groups , one with pico green and the other without at 488nm .<br> | |||
*Chip ran with total of 5ng of NA , pico green using the low range standard curve<br> | |||
Revision as of 12:22, 9 April 2009
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9 April 2009 Lab MeetingPressure sensor - quoted $325 SP70A or $1000+ SP70D, returning old one for them to look
STATUS? † Announcements
- nanopatterned COP provides access to repeatable morphology
- Do Rhodamine.
-Need a size specific positive control. PS beads? charged?
† RCA/HDA
† Silica Optimization (Lambda):
|