9 April 2009 Lab Meeting
† Announcements
- Need posters for Oakridge by Wed. morning.
† Flu R01
- Sonali is working on RTPCR and plaque assays for progress report.
† SEPSIS Project
†RNA project
- Need to get Jurkat RNA to Jeff. He may visit in May.
† COBRA
- Integration with the glass slide.
- Prototype next Wednesday.
- Get PMMA film.
- Substrates (JZ)
- Let's get this to a point where we can publish and move on....JZ and CMK to discuss.
- Colloid experiment.
- Do Rhodamine. Leaked. Need to try again.
-Need a size specific positive control. PS beads? charged?
- 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate
- try coated PS beads.
† Valve Array/Valve building - done.
† Fraunhofer: LOAC
- Paper submitted to LOC
- Bonding meeting with MIT folks - Alex left some samples with them.
† Biointerfaces group
- Cathie in charge of Bionterfaces Paper1. Cathie has final draft now before it goes to collaborators.
- designing herringbone groove for better trapping
- investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).
† CIMIT- Colson Grant
† PCR
- Cathie read and gave back PCR 1 paper to QQ and MCK
- Qingqing has written the introduction
- repeated test of the PCR efficiency for different designs of channel.
- test PCR efficiency using smaller DNA size (63bp product)
- analyze PCR product for different temperature using bioanalyzer.
- will make figures for PCR1 paper.
- developing large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)
† RCA/HDA
- Cathie submitted One pager Whitepaper. Waiting for response.
- convert this whitepaper to grant? RCA/HDA on a chip for NIH...anyone interested? ANYONE?
- Preliminary no-valve design for 25 microliters.
- 20 more test chips to Sonali (AACC poster).
- Sonali try to do 10 microliters in the tube
† Senior project
- Megan
- HDA on chip. Make simple chip that mixes two things for HDA. Move chip to heater, do HDA demonstration = home run.
- Plasma helped bonding.
- EHS wants MSDS stuff.
† F31: Cochlea
† Silica Optimization (Lambda):
- The following will be submitted monday
- total of 20 channels 10 as negative and 10 positive with 100ng total NA mass. Emission spectrum of negative samples with excitation at 488nm.
- Emission spectrum of negative samples at excitations corresponding to the NA sampler kit dyes
- Emission spectrum for the positive samples split into 2 groups , one with pico green and the other without at 488nm .
- Chip ran with total of 5ng of NA , pico green using the low range standard curve
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