Klapperich Lab:Notebook/Lab Meeting Notes/2009/05/07: Difference between revisions

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† COBRA <br>  
† COBRA <br>  
* Integration with the glass slide.  <br>
* Integration with the glass slide.  <br>
* Updates? <br>
* new air channel design sent to machine shop <br>
* new fluid channel made in PDMS <br>
* making the mold had proven challenging as it sticks to the plastic fixture, tried mold release but didn't help <br>
* new air channel mold did not cure well and the PDMS made from it was leaking. need to do it again, mold material ordered. <br>
<br>
<br>
* Substrates (JZ)<br>
* Substrates (JZ)<br>
Line 33: Line 36:
* Colloid experiment.<br>
* Colloid experiment.<br>
- Do Rhodamine. Leaked. Need to try again. <br>
- Do Rhodamine. Leaked. Need to try again. <br>
- Found the problem. Liquid and air channel widths reversed. Will make new chip and try again. <br>
** ordered <br>
** ordered <br>
-Need a size specific positive control. PS beads? charged? <br>
-Need a size specific positive control. PS beads? charged? <br>
** 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate <br>
** 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate <br>
** try coated PS beads. <br>
** try coated PS beads. <br>
<br>
† Virus concentration
* ATCC flu virus grew and harvested
* Plaque assay of harvested virus in progress: learning from Brendan, will try if enough cells
* concentrator chip design underway
<br>
<br>
† Valve Array/Valve building - done. <br>
† Valve Array/Valve building - done. <br>
Line 69: Line 80:
* 20 more test chips to Sonali (AACC poster). <br>
* 20 more test chips to Sonali (AACC poster). <br>
* Sonali try to do 10 microliters in the tube * Updates? <br>
* Sonali try to do 10 microliters in the tube * Updates? <br>
* Jane is working on passive valve characterization: pressure held not related to cantilever length or bonding time, try hole size next  <br>
<br>
<br>
† Senior project <br>
† Senior project <br>

Revision as of 10:28, 7 May 2009

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7 May 2009 Lab Meeting

† Announcements

  • New lab meeting time T 10a-12pm room 705 starting 5/12/2009


† Flu R01

  • Progress report done.
  • sample collection done.
    .
  • Updates?


† SEPSIS Project

  • Revision in process due 5/20


†RNA project

  • Jeff visiting week of May 25th


† COBRA

  • Integration with the glass slide.
  • new air channel design sent to machine shop
  • new fluid channel made in PDMS
  • making the mold had proven challenging as it sticks to the plastic fixture, tried mold release but didn't help
  • new air channel mold did not cure well and the PDMS made from it was leaking. need to do it again, mold material ordered.


  • Substrates (JZ)
    • Let's get this to a point where we can publish and move on....JZ and CMK to discuss.
  • Colloid experiment.

- Do Rhodamine. Leaked. Need to try again.
- Found the problem. Liquid and air channel widths reversed. Will make new chip and try again.

    • ordered

-Need a size specific positive control. PS beads? charged?

    • 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate
    • try coated PS beads.


† Virus concentration

  • ATCC flu virus grew and harvested
  • Plaque assay of harvested virus in progress: learning from Brendan, will try if enough cells
  • concentrator chip design underway


† Valve Array/Valve building - done.

  • Who is in charge of this now that the SP students are gone? Alex?

† Fraunhofer: LOAC

  • Paper in revision Yeah!
  • Bonding meeting with MIT folks - Alex left some samples with them. Updates?


† Biointerfaces group

  • Final draft with collaborators.
  • designing herringbone groove for better trapping (Mask writer down until next Tuesday)
  • investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).


† CIMIT- Colson Grant

  • IRB still in revision.


† PCR

  • Cathie read and gave back PCR 1 paper to QQ and MCK
  • Qingqing has written the introduction
  • repeated test of the PCR efficiency for different designs of channel.
  • test PCR efficiency using smaller DNA size (63bp product).
  • analyze PCR product for different temperature using bioanalyzer.
  • getting statistical data for the activation energy from 50 particle simulation (Fig. 5-7)
  • Mincheol has written the introduction, results and discussion, and conclusion.
  • will develop large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)


† RCA/HDA

  • Giving up on white paper. Think about other grant outlets.
  • Preliminary no-valve design for 25 microliters.
  • 20 more test chips to Sonali (AACC poster).
  • Sonali try to do 10 microliters in the tube * Updates?
  • Jane is working on passive valve characterization: pressure held not related to cantilever length or bonding time, try hole size next


† Senior project

  • Megan
  • HDA on chip. Make simple chip that mixes two things for HDA. Move chip to heater, do HDA demonstration = home run.
  • Plasma helped bonding.
  • EHS wants MSDS stuff.


† Silica Optimization (Lambda):

  • The following will be submitted monday


  • total of 20 channels 10 as negative and 10 positive with 100ng total NA mass. Emission spectrum of negative samples with excitation at 488nm.
  • Emission spectrum of negative samples at excitations corresponding to the NA sampler kit dyes
  • Emission spectrum for the positive samples split into 2 groups , one with pico green and the other without at 488nm .
  • Chip ran with total of 5ng of NA , pico green using the low range standard curve



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