Klapperich Lab:Notebook/Lab Meeting Notes/2009/05/07

7 May 2009 Lab Meeting

† Announcements

• New lab meeting time T 10a-12pm room 705 starting 5/12/2009
  

† Flu R01

• Progress report done.
  
• sample collection done.
  .
• Updates?
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† SEPSIS Project

• Revision in process due 5/20
  
• 
  

†RNA project

• Jeff visiting week of May 25th
  
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† COBRA

• Integration with the glass slide.
  
• new air channel design sent to machine shop
  
• new fluid channel made in PDMS
  
• making the polyurethane mold had proven challenging as it sticks to the plastic fixture, tried mold release but didn't help
  
• new cutting plotter carrier sheet ordered
  
• new air channel mold did not cure well and the PDMS made from it was leaking. need to do it again, mold material ordered.
  

• Substrates (JZ)
  
  • Let's get this to a point where we can publish and move on....JZ and CMK to discuss.
    

- Making substrate in microwewlls? (motivation? need valves)
- Colloidal experiment? (the morphology won't be uniform or consistent each time, signal analysis system non-existent)
- Sol-gel on Teflon? (can serve to study basic material science only)

• Colloid experiment
  

- Do Rhodamine. Leaked. Need to try again.
- Found the problem. Liquid and air channel widths reversed. Will make new chip and try again.
-Need a size specific positive control. PS beads? charged?

• • 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate
    
  • try coated PS beads.
    

† Virus concentration

• ATCC flu virus grew and harvested
• Plaque assay of harvested virus in progress: learning from Brendan, will try if enough cells
• Concentrator chip design underway

† Valve Array/Valve building - done.

• Who is in charge of this now that the SP students are gone? Alex?
  

† Fraunhofer: LOAC

• Paper in revision Yeah!
  
• Bonding meeting with MIT folks - Alex left some samples with them. Updates?
  

† Biointerfaces group

• Final draft with collaborators.
  
• designing herringbone groove for better trapping (Mask writer down until next Tuesday)
  
• investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).
  

† CIMIT- Colson Grant

• IRB still in revision.
  
• 
  

† PCR

• Cathie read and gave back PCR 1 paper to QQ and MCK
  
• Qingqing has written the introduction
  
• repeated test of the PCR efficiency for different designs of channel.
  
• test PCR efficiency using smaller DNA size (63bp product).
  
• analyze PCR product for different temperature using bioanalyzer.
  
• getting statistical data for the activation energy from 50 particle simulation (Fig. 5-7)
  
• Mincheol has written the introduction, results and discussion, and conclusion.
  
• will develop large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)
  
  

† RCA/HDA

• Giving up on white paper. Think about other grant outlets.
  
• Preliminary no-valve design for 25 microliters.
  
• 20 more test chips to Sonali (AACC poster).
  
• Sonali try to do 10 microliters in the tube * Updates?
  
• Jane is working on passive valve characterization: threshold pressure not related to cantilever length or bonding time, try hole size next
  

† Senior project

• Megan
  
• HDA on chip. Make simple chip that mixes two things for HDA. Move chip to heater, do HDA demonstration = home run.
  
• Plasma helped bonding.
  
• EHS wants MSDS stuff.
  

† Silica Optimization (Lambda):

• The following will be submitted monday
  

• total of 20 channels 10 as negative and 10 positive with 100ng total NA mass. Emission spectrum of negative samples with excitation at 488nm.
  
• Emission spectrum of negative samples at excitations corresponding to the NA sampler kit dyes
  
• Emission spectrum for the positive samples split into 2 groups , one with pico green and the other without at 488nm .
  
• Chip ran with total of 5ng of NA , pico green using the low range standard curve