7 May 2009 Lab Meeting
† Announcements
- New lab meeting time T 10a-12pm room 705 starting 5/12/2009
† Flu R01
- Progress report done.
- sample collection done.
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- Updates?
† SEPSIS Project
- Revision in process due 5/20
†RNA project
- Jeff visiting week of May 25th
† COBRA
- Integration with the glass slide.
- new air channel design sent to machine shop
- new fluid channel made in PDMS
- making the mold had proven challenging as it sticks to the plastic fixture, tried mold release but didn't help
- new cutting plotter carrier sheet ordered
- new air channel mold did not cure well and the PDMS made from it was leaking. need to do it again, mold material ordered.
- Substrates (JZ)
- Let's get this to a point where we can publish and move on....JZ and CMK to discuss.
- Making substrate in microwewlls? (motivation? need valves)
- Colloidal experiment? (the morphology won't be uniform or consistent each time, signal analysis system non-existent)
- Sol-gel on Teflon? (can serve to study basic material science only)
- Do Rhodamine. Leaked. Need to try again.
- Found the problem. Liquid and air channel widths reversed. Will make new chip and try again.
-Need a size specific positive control. PS beads? charged?
- 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate
- try coated PS beads.
† Virus concentration
- ATCC flu virus grew and harvested
- Plaque assay of harvested virus in progress: learning from Brendan, will try if enough cells
- Concentrator chip design underway
† Valve Array/Valve building - done.
- Who is in charge of this now that the SP students are gone? Alex?
† Fraunhofer: LOAC
- Paper in revision Yeah!
- Bonding meeting with MIT folks - Alex left some samples with them. Updates?
† Biointerfaces group
- Final draft with collaborators.
- designing herringbone groove for better trapping (Mask writer down until next Tuesday)
- investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).
† CIMIT- Colson Grant
† PCR
- Cathie read and gave back PCR 1 paper to QQ and MCK
- Qingqing has written the introduction
- repeated test of the PCR efficiency for different designs of channel.
- test PCR efficiency using smaller DNA size (63bp product).
- analyze PCR product for different temperature using bioanalyzer.
- getting statistical data for the activation energy from 50 particle simulation (Fig. 5-7)
- Mincheol has written the introduction, results and discussion, and conclusion.
- will develop large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)
† RCA/HDA
- Giving up on white paper. Think about other grant outlets.
- Preliminary no-valve design for 25 microliters.
- 20 more test chips to Sonali (AACC poster).
- Sonali try to do 10 microliters in the tube * Updates?
- Jane is working on passive valve characterization: pressure held not related to cantilever length or bonding time, try hole size next
† Senior project
- Megan
- HDA on chip. Make simple chip that mixes two things for HDA. Move chip to heater, do HDA demonstration = home run.
- Plasma helped bonding.
- EHS wants MSDS stuff.
† Silica Optimization (Lambda):
- The following will be submitted monday
- total of 20 channels 10 as negative and 10 positive with 100ng total NA mass. Emission spectrum of negative samples with excitation at 488nm.
- Emission spectrum of negative samples at excitations corresponding to the NA sampler kit dyes
- Emission spectrum for the positive samples split into 2 groups , one with pico green and the other without at 488nm .
- Chip ran with total of 5ng of NA , pico green using the low range standard curve
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