Klapperich Lab:Notebook/Lab Meeting Notes/2009/06/09: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Jane Zhang (talk | contribs) |
|||
| (9 intermediate revisions by one other user not shown) | |||
| Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== 9 June 2009 Lab Meeting== | == 9 June 2009 Lab Meeting== | ||
* attening: Hussam, Jaephil, Jane, MinCheol, Brendan, Jessie, QQ, Sonali, Me. | |||
Missing: Alex<br> | |||
* Jaephil gave small evap presentation to the group. <br> | |||
<br> | |||
† Announcements<br> | † Announcements<br> | ||
* | * Check on your NEW LAB DUTIES WITH SONALI Then do them and check off when they are done each week. <br> | ||
* Short small team meetings. 45 min -- SEE GOOGLE calendar for schedule <br> | * Short small team meetings. 45 min -- SEE GOOGLE calendar for schedule <br> | ||
Suggested groups... please edit. Teams<br> | Suggested groups... please edit. Teams<br> | ||
| Line 15: | Line 18: | ||
2. Bacteria = JZ, JD <br> | 2. Bacteria = JZ, JD <br> | ||
3. Flu = MM, JZ, HM, JC, BC <br> | 3. Flu = MM, JZ, HM, JC, BC <br> | ||
3. Sample Preparation = AG, HM? <br> | 3. HDA? Sample Preparation = AG, HM? <br> | ||
* MicroTAS Abstracts accepted. (4) Papers due June 30th. <br> | * MicroTAS Abstracts accepted. (4) Papers due June 30th. <br> | ||
<br> | <br> | ||
† Flu R01 <br> | † Flu R01 <br> | ||
* RNA extraction troubleshooting. <br> | * RNA extraction troubleshooting. <br> | ||
* Try clean fabrication of chips and monoliths.<br> | |||
* Try RNAseH wash through. <br> | |||
* Absolute Quantification assays: TH this week. <br> | * Absolute Quantification assays: TH this week. <br> | ||
* FTIR <br> | * FTIR/maybe next week. <br> | ||
* <br> | * Samples for Mass Spec. <br> | ||
* A primers look good at this point. Van Elden 2001. <br> | |||
* Jessie should learn SEM. <br> | |||
† Virus concentration (upstream from the assay.) <br> | |||
* QQ - HM - JZ - Chip Integration. <br> | |||
* How to read? Color, sensitivity? Alignment. Check with JD. <br> | |||
<br> | |||
† Coulter Flu Fraunhofer Project | † Coulter Flu Fraunhofer Project | ||
* Fraun folks coming to get trained next week. RNA isolation. <br> | * Fraun folks coming to get trained next week. RNA isolation. Safety issues. English. <br> | ||
* IBC for Fraun flu. | * IBC for Fraun flu. waiting? <br> | ||
* Brendan can come to Fraun Flu meetings if he wants....as primer design goes. <br> | |||
<br> | <br> | ||
† SEPSIS Project <br> | † SEPSIS Project <br> | ||
| Line 47: | Line 60: | ||
- Need to redefine hypothesis | - Need to redefine hypothesis | ||
<br> | <br> | ||
<br> | <br> | ||
† Valve Array <br> | † Valve Array <br> | ||
| Line 59: | Line 71: | ||
† Biointerfaces group <br> | † Biointerfaces group <br> | ||
* Meeting Wed 2-4pm. <br> | * Meeting Wed 2-4pm. <br> | ||
* Printed out paper for everyone. Go over the figures. Set the "story." <br> | |||
* fabricated SU-8 mold with Herringbone grooves. <br> | * fabricated SU-8 mold with Herringbone grooves. <br> | ||
* Brett | * Brett took a photo of the device for the paper, but will repeat <br> | ||
* Jason coated thin gold on the surface of the PDMS mold, and will take SEM images for the sieves. <br> | * Jason coated thin gold on the surface of the PDMS mold, and will take SEM images for the sieves. --->SEM down<br> | ||
* draft paper2 | * draft paper2 for the submission to "Biophysical Journal" has been started. <br> | ||
* Will repeat trapping experiments this Friday to obtain good quality images for the Fig. 2 in the paper. <br> | |||
<br> | <br> | ||
† CIMIT- Colson Grant<br> | † CIMIT- Colson Grant<br> | ||
| Line 69: | Line 83: | ||
† PCR <br> | † PCR <br> | ||
* PCR 2 paper draft. <br> | * QQ: PCR 2 paper draft. <br> | ||
* PEG-coating protocol developed | * CMK: PCR 1 draft <br> | ||
* PEG-coating protocol developed - TROUBLESHOOTING. <br> | |||
* on-chip experiment with blank chip without PEG,BSA. It works with expected lower efficiency <br> | * on-chip experiment with blank chip without PEG,BSA. It works with expected lower efficiency <br> | ||
* on-chip experiment with PEG-coated chip without PEG,BSA in reagent. It does not work, or the product is under the detection limitation of bioanalyzer. <br> trouble-shooting... | * on-chip experiment with PEG-coated chip without PEG,BSA in reagent. It does not work, or the product is under the detection limitation of bioanalyzer. <br> trouble-shooting...<br> | ||
* QQ : write protocol for PEG graft. <br> | |||
* QQ: Try changing heater and thermocouple. <br> | |||
* QQ: Try running PCR after plasma treatment and water wash only. <br> | |||
* QQ and MM: look back at flu PCR. Recall the repeatability. Decide course of action with Sonali. See what you can do to do "real" samples soon. One pot? Two steps necessary instead? <br> | |||
<br> | |||
† RCA/HDA<br> | † RCA/HDA<br> | ||
* Getting solution back out is still an issue. <br> | * Getting solution back out is still an issue. <br> | ||
Revision as of 21:11, 15 June 2009
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
9 June 2009 Lab Meeting
Missing: Alex
Suggested groups... please edit. Teams
† Virus concentration (upstream from the assay.)
†RNA project
† COBRA
paper 1: Evap with Sol Gel substrate.
† PCR
† Silica Optimization (Lambda):
| |
