Klapperich Lab:Notebook/Lab Meeting Notes/2009/06/09

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(9 June 2009 Lab Meeting)
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† Biointerfaces group <br>
† Biointerfaces group <br>
* Meeting Wed 2-4pm. <br>
* Meeting Wed 2-4pm. <br>
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* found a method of double stacking SU-8 using mask-aligner for making Herringbone grooves. <br>
+
* fabricated SU-8 mold with Herringbone grooves. <br>
-
* investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).<br>
+
* Brett will take a photo of the device for the paper. <br>
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* asked Brett to take a photo of the device for the paper. <br>
+
* Jason coated thin gold on the surface of the PDMS mold, and will take SEM images for the sieves. <br>
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* MC and Jason will get SEM images of PDMS mold with thin gold coating of nano-size on its surface. <br>
+
* draft paper2 will be started soon. <br>  
* draft paper2 will be started soon. <br>  
<br>
<br>

Revision as of 00:40, 6 June 2009

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9 June 2009 Lab Meeting

† Announcements

  • SIGN UP FOR NEW LAB DUTIES WITH SONALI.
  • Short small team meetings. 45 min -- SEE GOOGLE calendar for schedule

Suggested groups... please edit. Teams
1. PCR= QQ, MCK, MM
2. Bacteria = JZ, JD
3. Flu = MM, JZ, HM, JC, BC
3. Sample Preparation = AG, HM?

  • MicroTAS Abstracts accepted. (4) Papers due June 30th.


† Flu R01

  • RNA extraction troubleshooting.
  • Absolute Quantification assays: TH this week.
  • FTIR

† Coulter Flu Fraunhofer Project

  • Fraun folks coming to get trained next week. RNA isolation.
  • IBC for Fraun flu. approved?


† SEPSIS Project

  • Revision submitted 5/20.

†RNA project

  • Call Natalia.

† COBRA

  • Test with sol-gel substrate today(2nd June)
  • delayed due to difficulty adjusting membrane thickness
  • Evap system (JD and JZ)


    • Let's get this to a point where we can publish and move on

paper 1: Evap with Sol Gel substrate.
paper 2: Covap with PMA, Rhodamine, PS beads?...virus...?

† Virus concentration



† Valve Array

  • Teddy will give training presentation(guide).


† Fraunhofer: LOAC

  • Paper revised.
  • Bonding ongoing with MIT folks


† Biointerfaces group

  • Meeting Wed 2-4pm.
  • fabricated SU-8 mold with Herringbone grooves.
  • Brett will take a photo of the device for the paper.
  • Jason coated thin gold on the surface of the PDMS mold, and will take SEM images for the sieves.
  • draft paper2 will be started soon.


† CIMIT- Colson Grant

  • IRB still in revision.

† PCR

  • PCR 2 paper draft.
  • PEG-coating protocol developed
  • on-chip experiment with blank chip without PEG,BSA. It works with expected lower efficiency
  • on-chip experiment with PEG-coated chip without PEG,BSA in reagent. It does not work, or the product is under the detection limitation of bioanalyzer.
    trouble-shooting...

† RCA/HDA

  • Getting solution back out is still an issue.
  • Integrated chip worked (19th). Accumulating multiple runs. (Check into primer lifetime)
  • Evaporation problems near edges. Maybe design change?
  • Teflon issue with the enzyme? Check into it.

† Silica Optimization (Lambda):


  • Get lower Bioan. Kit.



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