Klapperich Lab:Notebook/Lab Meeting Notes/2009/07/07: Difference between revisions
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== 7 July 2009 Lab Meeting== | |||
* Attending: <br> | |||
* Presentation: ?? <br> | |||
<br> | |||
† Announcements<br> | |||
*<br> | |||
<br> | |||
† Flu R01 <br> | |||
* Signed CDC MTA. <br> | |||
* Integration: Spec? On chip? Jane will look into it. <br> | |||
* QQ will work on the initial integration steps of SPE + RT (resevoir)+ PCR. Cutter plotter? Faunhofer Chip serpentine channel. <br> | |||
* RNA extraction troubleshooting. <br> | |||
* Try clean fabrication of chips and monoliths.<br> | |||
* Try RNAseH wash through. <br> | |||
* Absolute Quantification assays: <br> | |||
* A primers look good at this point. Van Elden 2001. <br> | |||
* Jessie should learn SEM. <br> | |||
† Virus concentration (upstream from the assay.) <br> | |||
* QQ - HM - JZ - Chip Integration. <br> | |||
* How to read? Color, sensitivity? Alignment. Check with JD. <br> | |||
<br> | |||
† Coulter Flu Fraunhofer Project | |||
* Fraun folks coming to get trained next week. RNA isolation. Safety issues. English. <br> | |||
* IBC for Fraun flu. waiting? <br> | |||
* Brendan can come to Fraun Flu meetings if he wants....as primer design goes. <br> | |||
* Ajani should come to Fraun Flu meetings to learn how to make straws. Alex will train him. <br> | |||
<br> | |||
'''† SEPSIS Project <br> | |||
* Published! <br>''' | |||
* <br> | |||
†RNA project | |||
* Call Natalia. <br> | |||
* <br> | |||
† COBRA <br> | |||
* Update on concentration of live bugs? <br> | |||
* Evap system (JD and JZ)<br> | |||
<br> | |||
** Let's get this to a point where we can publish and move on | |||
paper 1: Evap with Sol Gel substrate. <br> | |||
paper 2: Covap with PMA, Rhodamine, PS beads?...virus...?<br> | |||
- Rhodamine experiments done <br> | |||
- Signals are similar between with and without GNP <br> | |||
- Rhodamine binds poorly to gold <br> | |||
- try GNP-pMA next. R6G-silver NP. | |||
- Need to redefine hypothesis | |||
<br> | |||
<br> | |||
† Biointerfaces group <br> | |||
* fabricated SU-8 mold with Herringbone grooves. <br> | |||
* Brett took a photo of the device for the paper, but will repeat <br> | |||
* Jason coated thin gold on the surface of the PDMS mold, and will take SEM images for the sieves. --->SEM down<br> | |||
* draft paper2 for the submission to "Biophysical Journal" has been started. <br> | |||
* Will repeat trapping experiments this Friday to obtain good quality images for the Fig. 2 in the paper. <br> | |||
<br> | |||
† CIMIT- Colson Grant<br> | |||
* IRB submitted.<br> | |||
* Cathie working on the companion BU IRB form for exemption. <br> | |||
<br> | |||
† PCR <br> | |||
* QQ: PCR 2 paper draft. <br> | |||
* CMK: PCR 1 draft <br> | |||
* DONE with PEG attempts. <br> | |||
* PEG-coating protocol developed - TROUBLESHOOTING. <br> | |||
* on-chip experiment with blank chip without PEG,BSA. It works with expected lower efficiency <br> | |||
* on-chip experiment with PEG-coated chip without PEG,BSA in reagent. It does not work, or the product is under the detection limitation of bioanalyzer. | |||
* QQ : write protocol for PEG graft. <br> | |||
it has been uploaded in the share driver. | |||
* QQ: Try changing heater and thermocouple. <br> | |||
done | |||
* QQ: Try running PCR after plasma treatment and water wash only. <br> | |||
done | |||
* QQ and MM: look back at flu PCR. Recall the repeatability. Decide course of action with Sonali. See what you can do to do "real" samples soon. One pot? Two steps necessary instead? <br> | |||
the repeatebility has been comfirmed. | |||
<br> | |||
† RCA/HDA<br> | |||
* Start planning R01 for Submission on 10/5/09. MM, JD, CMK. <br> | |||
* Getting solution back out is still an issue. <br> | |||
* Integrated chip worked (19th). Accumulating multiple runs. (Check into primer lifetime) <br> | |||
* Evaporation problems near edges. Maybe design change? <br> | |||
* Teflon issue with the enzyme? Check into it. <br> | |||
† Silica Optimization (Lambda): <br> | |||
* Do absolute quant assay. Mass Spec data suggests junk is polymer. Pursue GPC later. <br> | |||
* '''Get lower Bioan. Kit.''' <br> | |||
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7 July 2009 Lab Meeting
† Virus concentration (upstream from the assay.)
†RNA project
† COBRA
paper 1: Evap with Sol Gel substrate.
it has been uploaded in the share driver.
done
done
the repeatebility has been comfirmed.
† Silica Optimization (Lambda):
|