Klapperich Lab:Notebook/Lab Meeting Notes/2009/08/25: Difference between revisions

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* Try clean fabrication of chips and monoliths.<br>
* Try clean fabrication of chips and monoliths.<br>
* Try RNAseH wash through. <br>
* Try RNAseH wash through. <br>
* A primers look good at this point. Van Elden 2001. '''(C.L. Ward 2004 works best for PCR)'''<br>
* A primers look good at this point. Van Elden 2001. '''(C.L. Ward 2004 primers works best for PCR)'''<br>
* Jessie should learn SEM. <br>
* Jessie should learn SEM. <br>
'''* Need to update IBC to include rDNA work. <br>'''
'''* Need to update IBC to include rDNA work. <br>'''

Revision as of 13:29, 24 August 2009

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25 August 2009 Lab Meeting

  • Attending:
  • Missing:
  • Presentation: Jaephil: Chip Integration FLU


† Announcements

  • Lab lunch today after meeting.

† Flu R01

  • Do std curve without carrier RNA. Compare with ASB data.
  • Integration: Spec? On chip? Jane will look into it.
  • QQ will work on the initial integration steps of SPE + RT (resevoir)+ PCR. Cutter plotter? Faunhofer Chip serpentine channel. "QQ Needs to know how to make valves."
  • RNA extraction troubleshooting.
  • Try clean fabrication of chips and monoliths.
  • Try RNAseH wash through.
  • A primers look good at this point. Van Elden 2001. (C.L. Ward 2004 primers works best for PCR)
  • Jessie should learn SEM.

* Need to update IBC to include rDNA work.

† Virus concentration (upstream from the assay.)

  • How to read? Color, sensitivity? Alignment. Check with JD.


† Coulter Flu Fraunhofer Project

  • IBC for Fraun flu. approved.


† Agilent Meetings

  • With Straws in Parallel at this point.

† COBRA

  • Update on concentration of live bugs? MSSA?
  • Evap system (JD and JZ)


    • Let's get this to a point where we can publish and move on

paper 1: Evap with Sol Gel substrate.
paper 2: Covap with PMA, Rhodamine, PS beads?...virus...?
- Rhodamine experiments done
- Signals are similar between with and without GNP
- Rhodamine binds poorly to gold
- try GNP-pMA next. R6G-silver NP. - Need to redefine hypothesis

† Biointerfaces group

  • New fabrcation in process.


† CIMIT- Colson Grant

  • IRB approved.
  • Cathie submitted the companion BU IRB form for exemption.


† PCR

  • QQ: PCR 2 paper draft.
  • CMK: PCR 1 draft

* on-chip PCR on a series of diluted lambda phage DNA. 

 the detect limitation for ABI is 10-9g/ul
 on-chip PCR have the same sensitivity.
  • With new syring and tubing, on-chip PCR for the patient sample does not have detectable amplified product. however, the postitive control on the thermal cycle works good.

† RCA/HDA

  • Start planning R01 for Submission on 10/5/09. MM, JD, CMK.
  • Getting solution back out is still an issue.
  • Integrated chip worked (19th). Accumulating multiple runs. (Check into primer lifetime)
  • Evaporation problems near edges. Maybe design change?
  • Teflon issue with the enzyme? Check into it.

† Silica Optimization (Lambda):

  • St. Curve for Abs Quant Assay.



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