Klapperich Lab:Notebook/Lab Meeting Notes/2009/08/25: Difference between revisions
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* took SEM images of parallelized designs, will show them if possible in the meeting. <br> | * took SEM images of parallelized designs, will show them if possible in the meeting. <br> | ||
* Through SEM images, we found the smallest feature size is 1.2 um. <br> | * Through SEM images, we found the smallest feature size is 1.2 um. <br> | ||
* set up 2 week check in meeting with team. <br> | |||
<br> | <br> | ||
† CIMIT- | † CIMIT- Sepsis<br> | ||
* IRB approved. <br> | * IRB approved. <br> | ||
* Cathie submitted the companion BU IRB form for exemption. <br> | * Cathie submitted the companion BU IRB form for exemption. <br> | ||
<br> | <br> | ||
† PCR <br> | † PCR (will get absorbed into the Flu agenda item at the top) <br> | ||
* | * CMK: PCR 2 paper draft. <br> | ||
* CMK: PCR 1 draft <br> | * CMK: PCR 1 draft. Analytical Chem. <br> | ||
'''* on-chip PCR on a series of diluted lambda phage DNA.''' | '''* on-chip PCR on a series of diluted lambda phage DNA.''' | ||
''' the detect limitation for ABI is 10<sup>-9</sup>g/ul | ''' the detect limitation for ABI is 10<sup>-9</sup>g/ul | ||
on-chip PCR have the same sensitivity.''' | on-chip PCR have the same sensitivity.''' | ||
'''* With new syring and tubing, on-chip PCR for the patient sample does not have detectable amplified product. however, the postitive control on the thermal cycle works good.''' | '''* With new syring and tubing, on-chip PCR for the patient sample does not have detectable amplified product. however, the postitive control on the thermal cycle works good.''' <br> | ||
* New primers? <br> | |||
* Double PCR on chip. <br> | |||
<br> | |||
† RCA/HDA<br> | † RCA/HDA<br> | ||
* Start planning R01 for Submission on | * Start planning R01 for Submission on 2/5/10. MM, JD, CMK. <br> | ||
* | * JD working on paper. Will deliver to MM this week. <br> | ||
* | * Figures discussion. <br> | ||
* | <br> | ||
* | * PATH Grant <br> | ||
* Frank J. | |||
* 12 mos, 24 mos. working prototypes of SNAP. <br> | |||
<br> | <br> | ||
† Silica Optimization (Lambda): <br> | † Silica Optimization (Lambda): (get absorbed into the flu agenda item at top) <br> | ||
* August-18-presentation [http://openwetware.org/wiki/Image:Aug_18_09_presentation.ppt] | * August-18-presentation [http://openwetware.org/wiki/Image:Aug_18_09_presentation.ppt] | ||
* Results Summary of Aug-18-09 [http://openwetware.org/wiki/Image:Results_Summary_Aug-18-09.xls] <br> | * Results Summary of Aug-18-09 [http://openwetware.org/wiki/Image:Results_Summary_Aug-18-09.xls] <br> |
Revision as of 08:14, 25 August 2009
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25 August 2009 Lab Meeting
† Flu R01
* Need to update IBC to include rDNA work.
The current problem is not how to visualize virus right now, because the design is clearly not optimized for collecting all the samples at the outlet. The major loss is from sample handling instead of from non specific binding to the material. † Coulter Flu Fraunhofer Project (minor item on new agenda)
* set up 2 week check in meeting with team.
* on-chip PCR on a series of diluted lambda phage DNA. the detect limitation for ABI is 10-9g/ul on-chip PCR have the same sensitivity. * With new syring and tubing, on-chip PCR for the patient sample does not have detectable amplified product. however, the postitive control on the thermal cycle works good.
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