Klapperich Lab:Notebook/Lab Meeting Notes/2009/08/25: Difference between revisions
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* How to read? Color, sensitivity? Alignment. Check with JD. <br> | * How to read? Color, sensitivity? Alignment. Check with JD. <br> | ||
'''Alexa Fluo-488 succinimidyl ester (Molecular Probes) stains the flu virus fluorescent. <br> | '''Alexa Fluo-488 succinimidyl ester (Molecular Probes) stains the flu virus fluorescent. <br> | ||
'''The current problem is not how to visualize virus right now, because the design is clearly not optimized for collecting all the samples | '''The current problem is not how to visualize virus right now, because the design is clearly not optimized for collecting all the samples at the outlet. The major loss is from sample handling instead of from non specific binding to the material. <br>''' | ||
'''My question about the this module of the integrated chip include: <br>''' | '''My question about the this module of the integrated chip include: <br>''' | ||
'''1. Fluid handling capacity. How much is the input fluid volume? As far as I heard, it makes more sense to concentrate mL's of patient sample to 100's uL, instead of from 100's uL to a few uL's. Is this true? <br> | '''1. Fluid handling capacity. How much is the input fluid volume? As far as I heard, it makes more sense to concentrate mL's of patient sample to 100's uL, instead of from 100's uL to a few uL's. Is this true? <br> | ||
'''2. Channel re-design. My major concern is the difficulty in fluid manipulation with pressure in the evaporation chip. ie. we cannot push the fluid with syringe pump, or collect the fluid with | '''2. Channel re-design. My major concern is the difficulty in fluid manipulation with pressure in the evaporation chip. ie. we cannot push the fluid with syringe pump, or collect the fluid with pipette in the liquid layer. Redesign of the channels involves incorporation of valves and direct flow of concentrated sample to the SPE module to minimize loss.<br>''' | ||
'''3. Materials. The chip should be made of COP, Teflon membrane, and Teflon film, so as to integrate well with the other modules. Therefore, the | '''3. Materials. The chip should be made of COP, Teflon membrane, and Teflon film, so as to integrate well with the other modules. Therefore, the air flow channel needs to be redesigned to make multi-layer fluid flow possible. <br> ''' | ||
† Coulter Flu Fraunhofer Project | † Coulter Flu Fraunhofer Project |
Revision as of 00:31, 25 August 2009
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25 August 2009 Lab Meeting
† Flu R01
Are we still looking to do this on chip? For on chip, we need to fabricate mirrors such that light can access assays in channels. For off chip, we need to look into OEM light source, fiber optices, fluorescence spectrometer, and detectors. They are pricey. such as this one: http://www.oceanoptics.com/products/usb4000fl.asp
* Need to update IBC to include rDNA work. † Virus concentration (upstream from the assay.)
Alexa Fluo-488 succinimidyl ester (Molecular Probes) stains the flu virus fluorescent. † Coulter Flu Fraunhofer Project
† COBRA
paper 1: Evap with Sol Gel substrate.
* on-chip PCR on a series of diluted lambda phage DNA. the detect limitation for ABI is 10-9g/ul on-chip PCR have the same sensitivity. * With new syring and tubing, on-chip PCR for the patient sample does not have detectable amplified product. however, the postitive control on the thermal cycle works good. † RCA/HDA
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