Klapperich Lab:Notebook/Lab Meeting Notes/2009/08/25: Difference between revisions
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* CMK: PCR 1 draft <br> | * CMK: PCR 1 draft <br> | ||
'''* on-chip PCR on a series of diluted lambda phage DNA.''' | '''* on-chip PCR on a series of diluted lambda phage DNA.''' | ||
''' the detect limitation for ABI is 10< | ''' the detect limitation for ABI is 10<sup>-9</sup>g/ul | ||
on-chip PCR have the same sensitivity.''' | on-chip PCR have the same sensitivity.''' | ||
'''* With new syring and tubing, on-chip PCR for the patient sample does not have detectable amplified product. however, the postitive control on the thermal cycle works good.''' | '''* With new syring and tubing, on-chip PCR for the patient sample does not have detectable amplified product. however, the postitive control on the thermal cycle works good.''' | ||
† RCA/HDA<br> | † RCA/HDA<br> | ||
* Start planning R01 for Submission on 10/5/09. MM, JD, CMK. <br> | * Start planning R01 for Submission on 10/5/09. MM, JD, CMK. <br> |
Revision as of 13:32, 24 August 2009
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25 August 2009 Lab Meeting
† Flu R01
* Need to update IBC to include rDNA work. † Virus concentration (upstream from the assay.)
† COBRA
paper 1: Evap with Sol Gel substrate.
* on-chip PCR on a series of diluted lambda phage DNA. the detect limitation for ABI is 10-9g/ul on-chip PCR have the same sensitivity. * With new syring and tubing, on-chip PCR for the patient sample does not have detectable amplified product. however, the postitive control on the thermal cycle works good. † RCA/HDA
† Silica Optimization (Lambda):
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