Klapperich Lab:Notebook/Lab Meeting Notes/2009/10/13: Difference between revisions
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== 13 October 2009 Lab Meeting== | |||
* Attending: <br> | |||
* Missing:<br> | |||
* Presentation: Dr. Qian Mei, Post Doc Applicant, 2pm, please BE ON TIME. <br> | |||
<br> | |||
'''‡ Announcements'''<br> | |||
* <br> | |||
* Invited speaker at group meeting on 10/20 - PLEASE Do NOT MISS - Dr. Tushar Bansal, post doc applicant. <br> | |||
* '''PAPERS Drafted before MicroTAS''' <br> | |||
* '''Posters for MicroTAS Due to CMK by 10/15'''<br> | |||
<br> | |||
'''‡ Flu R01:Integration''' <br> | |||
* New design - address evaporation loss, Reagent delivery and storage solution. Consider marginating flows? <br> | |||
<br> | |||
† Sample Concentration (Lead: Jane, Team: Jaephil) <br> | |||
* Up to high 10^7 for positive control. Silver substrate no signal. <br> | |||
* Jane working on the cell lysate control: plan to have Cassidy's help on plaque assay while I'm away. <br> | |||
* New cell passage from frozen - the old one is beyond passage 30. <br> | |||
* Main loss is at the outlet/sample collection: tangential filtration design in drawing stage. <br> | |||
* Contacted EC Shaw about rubber stamp mold for new design for evaporation. <br> | |||
* Set up COMSOL and StarCD, look for governing equations for simulation of evaporation. <br> | |||
* Committee meeting setup <br> | |||
<br> | |||
† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | |||
* Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between. Deliver CAD drawings for these for photomasks by Friday 10/16, functional chips to be delivered by 10/26 [Hussam/Jessie] | |||
* Silica free SPE, and SPE free channel experiment to be repeated. Control was old didn't take pressure, report back on 10/15.<br> | |||
* Load DNA in a straw of 50ul SPE, quick test of hypothesis more SPE = more DNA, report back 10/16. <br> | |||
<br> | |||
† PCR - CMI (Lead: Qingqing) <br> | |||
* QQ will work on the initial integration steps of SPE + RT (resevoir)+ PCR. <br> | |||
* PCR2 Paper formatted for LOAC this week. <br> | |||
* CMK: PCR 1 draft. Analytical Chem. MCK is running more simulations. <br> | |||
<br> | |||
† HDA (Lead: Jaephil, Team:Sonali)<br> | |||
* Start planning R01 for Submission on 2/5/10. MM, JD, CMK. <br> | |||
* JD will train QQ how to make HDA chips. This may entail moving from cutter plotter to embossing steps. JD and QQ to meet ASAP to plan the tech transfer. <br> | |||
* Paper submitted 10/6. <br> | |||
<br> | |||
'''‡C. diff Project''' (Cathie, Sonali, Satish Singh, Lisa J., His post doc )<br> | |||
* Sonali to train Lisa on SPE. <br> | |||
* QQ to run test PCR on chip with genomic DNA and Toxin B primers. <br> | |||
<br> | |||
'''‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing)''' | |||
* Meeting 9am Monday (every other week) <br> | |||
<br> | |||
'''‡ Agilent Automated Sample Preparation (Lead: Alex)'''<br> | |||
* With Straws in Parallel at this point. Speeding up the assay. <br> | |||
* Alex will have drafts this week. <br> | |||
<br> | |||
'''‡ COBRA (Lead: Jaephil, Team: Cathie, Jane for virus only, PHO folks)<br>''' | |||
* Metal piece modification for the integration - Parts distored and is begin fixed.<br> | |||
* paper 1: Evap with Sol Gel substrate. Not integrated. MSSA, E coli. <br> | |||
<br> | |||
'''‡ Biointerfaces group (Lead: MinCheol, Team: Cathie, MCK, Wong and Meller folks)'''<br> | |||
* New experiments happening now. <br> | |||
* Cathie will submit paper inquiry.<br> | |||
<br> | |||
'''‡ CIMIT- Sepsis (Lead:Cathie, Team:TBA)'''<br> | |||
* IRB approved. <br> | |||
* Cathie submitted the companion BU IRB form for exemption. Revised, still still waiting. <br> | |||
<br> | |||
'''‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME), Suma)''' <br> | |||
* Phone meeting with PATH this week. 10/13. <br> | |||
* Training on straw making concluded. <br> | |||
* UGs purchasing parts for straw machine for 709. <br> | |||
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13 October 2009 Lab Meeting
† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan)
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