Klapperich Lab:Notebook/Lab Meeting Notes/2009/10/13: Difference between revisions
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'''‡ Announcements'''<br> | '''‡ Announcements'''<br> | ||
* | * <br> | ||
* Invited speaker at group meeting on 10/ | * Invited speaker at group meeting on 10/20 - PLEASE Do NOT MISS - Dr. Tushar Bansal, post doc applicant. <br> | ||
* '''PAPERS Drafted before MicroTAS''' <br> | * '''PAPERS Drafted before MicroTAS''' <br> | ||
* '''Posters for MicroTAS Due to CMK by 10/15'''<br> | * '''Posters for MicroTAS Due to CMK by 10/15'''<br> | ||
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† Sample Concentration (Lead: Jane, Team: Jaephil) <br> | † Sample Concentration (Lead: Jane, Team: Jaephil) <br> | ||
* Up to 10^ | * Up to high 10^7 for positive control. Silver substrate no signal. <br> | ||
* Jane working on the cell lysate control. <br> | * Jane working on the cell lysate control: plan to have Cassidy's help on plaque assay while I'm away. <br> | ||
* Main loss is at the outlet/sample collection. <br> | * New cell passage from frozen - the old one is beyond passage 30. <br> | ||
* | * Main loss is at the outlet/sample collection: tangential filtration design in drawing stage. <br> | ||
* | * Contacted EC Shaw about rubber stamp mold for new design for evaporation. <br> | ||
* | * Set up COMSOL and StarCD, look for governing equations for simulation of evaporation. <br> | ||
* Committee meeting setup <br> | |||
<br> | <br> | ||
† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | † SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | ||
* | * Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between. Deliver CAD drawings for these for photomasks by Friday 10/16, functional chips to be delivered by 10/26 [Hussam/Jessie] | ||
* Silica free SPE, and SPE free channel experiment to be repeated. Control was old didn't take pressure, report back on 10/15.<br> | |||
* Load DNA in a straw of 50ul SPE, quick test of hypothesis more SPE = more DNA, report back 10/16. <br> | |||
*Silica free SPE, and SPE free channel experiment | |||
* | |||
<br> | <br> | ||
† PCR - CMI (Lead: Qingqing) <br> | † PCR - CMI (Lead: Qingqing) <br> | ||
* QQ will work on the initial integration steps of SPE + RT (resevoir)+ PCR. <br> | * QQ will work on the initial integration steps of SPE + RT (resevoir)+ PCR. <br> | ||
* | * PCR2 Paper formatted for LOAC this week. <br> | ||
* CMK: PCR 1 draft. Analytical Chem. MCK is running more simulations. <br> | * CMK: PCR 1 draft. Analytical Chem. MCK is running more simulations. <br> | ||
<br> | <br> | ||
† HDA (Lead: Jaephil, Team:Sonali)<br> | † HDA (Lead: Jaephil, Team:Sonali)<br> | ||
* Start planning R01 for Submission on 2/5/10. MM, JD, CMK. <br> | * Start planning R01 for Submission on 2/5/10. MM, JD, CMK. <br> | ||
* JD will train QQ how to make HDA chips. This may entail moving from cutter plotter to embossing steps. JD and QQ to meet ASAP to plan the tech transfer. <br> | |||
* Paper submitted 10/6. <br> | * Paper submitted 10/6. <br> | ||
<br> | <br> | ||
'''‡C. diff Project''' (Cathie, Sonali, Satish Singh, His post doc )<br> | '''‡C. diff Project''' (Cathie, Sonali, Satish Singh, Lisa J., His post doc )<br> | ||
* Sonali to train Lisa on SPE. <br> | * Sonali to train Lisa on SPE. <br> | ||
* QQ to run test PCR on chip with genomic DNA and Toxin B primers. <br> | * QQ to run test PCR on chip with genomic DNA and Toxin B primers. <br> | ||
<br> | <br> | ||
'''‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing)''' | '''‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing)''' | ||
* Meeting 9am Monday | * Meeting 9am Monday (every other week) <br> | ||
<br> | <br> | ||
'''‡ Agilent Automated Sample Preparation (Lead: Alex)'''<br> | '''‡ Agilent Automated Sample Preparation (Lead: Alex)'''<br> | ||
* With Straws in Parallel at this point. Speeding up the assay. <br> | * With Straws in Parallel at this point. Speeding up the assay. <br> | ||
* Alex will have drafts this week. <br> | |||
* Alex | |||
<br> | <br> | ||
'''‡ COBRA (Lead: Jaephil, Team: Cathie, Jane for virus only, PHO folks)<br>''' | '''‡ COBRA (Lead: Jaephil, Team: Cathie, Jane for virus only, PHO folks)<br>''' | ||
* | * Metal piece modification for the integration - Parts distored and is begin fixed.<br> | ||
* paper 1: Evap with Sol Gel substrate. Not integrated. MSSA, E coli. <br> | * paper 1: Evap with Sol Gel substrate. Not integrated. MSSA, E coli. <br> | ||
<br> | <br> | ||
'''‡ Biointerfaces group (Lead: MinCheol, Team: Cathie, MCK, Wong and Meller folks)'''<br> | '''‡ Biointerfaces group (Lead: MinCheol, Team: Cathie, MCK, Wong and Meller folks)'''<br> | ||
* New experiments happening now. <br> | * New experiments happening now. <br> | ||
* Cathie will submit paper inquiry.<br> | |||
<br> | <br> | ||
'''‡ CIMIT- Sepsis (Lead:Cathie, Team:TBA)'''<br> | '''‡ CIMIT- Sepsis (Lead:Cathie, Team:TBA)'''<br> | ||
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'''‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME), Suma)''' <br> | '''‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME), Suma)''' <br> | ||
* Phone meeting with PATH this week. <br> | * Phone meeting with PATH this week. 10/13. <br> | ||
* Training on straw making concluded. <br> | * Training on straw making concluded. <br> | ||
* UGs purchasing parts for straw machine for 709. <br> | |||
Revision as of 17:05, 13 October 2009
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13 October 2009 Lab Meeting
† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan)
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