Klapperich Lab:Notebook/Lab Meeting Notes/2009/10/13: Difference between revisions

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'''‡ Announcements'''<br>
'''‡ Announcements'''<br>
* No group meeting 10/6. <br>
* <br>
* Invited speaker at group meeting on 10/13 - PLEASE Do NOT MISS  - Dr. Qian Mei, post doc applicant. <br>
* Invited speaker at group meeting on 10/20 - PLEASE Do NOT MISS  - Dr. Tushar Bansal, post doc applicant. <br>
* '''PAPERS Drafted before MicroTAS''' <br>
* '''PAPERS Drafted before MicroTAS''' <br>
* '''Posters for MicroTAS Due to CMK by 10/15'''<br>
* '''Posters for MicroTAS Due to CMK by 10/15'''<br>
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† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br>
† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br>
* RNA extraction troubleshooting. Do deinhibition of monolith components. Test those with the Ambion RNAse kit. Jessie this week. <br>
* Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between. Deliver CAD drawings for these for photomasks by Friday 10/16, functional chips to be delivered by 10/26 [Hussam/Jessie]
* Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between. Deliver CAD drawings for these for photomasks by Friday 10/16, functional chips to be delivered by 10/26 [hussam/Jessie]
* Silica free SPE, and SPE free channel experiment to be repeated. Control was old didn't take pressure, report back on 10/15.<br>
*Silica free SPE, and SPE free channel experiment to be repeated. Control was old didn't take pressure, report back on 10/15.
* Load DNA in a straw of 50ul SPE, quick test of hypothesis more SPE = more DNA, report back 10/16.  <br>
*Load DNA in a straw of 50ul SPE, quick test of hypothesis more SPE = more DNA, report back 10/16.   
* Jessie worked with plasmid. Good std. curve. Not good predictor of the real sample amount. J will redo math with Sonali and Hussam to check.<br>
<br>
<br>
† PCR - CMI  (Lead: Qingqing) <br>
† PCR - CMI  (Lead: Qingqing) <br>

Revision as of 16:57, 13 October 2009

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13 October 2009 Lab Meeting

  • Attending:
  • Missing:
  • Presentation: Dr. Qian Mei, Post Doc Applicant, 2pm, please BE ON TIME.


‡ Announcements


  • Invited speaker at group meeting on 10/20 - PLEASE Do NOT MISS - Dr. Tushar Bansal, post doc applicant.
  • PAPERS Drafted before MicroTAS
  • Posters for MicroTAS Due to CMK by 10/15


‡ Flu R01:Integration

  • New design - address evaporation loss, Reagent delivery and storage solution. Consider marginating flows?


† Sample Concentration (Lead: Jane, Team: Jaephil)

  • Up to high 10^7 for positive control. Silver substrate no signal.
  • Jane working on the cell lysate control: plan to have Cassidy's help on plaque assay while I'm away.
  • New cell passage from frozen - the old one is beyond passage 30.
  • Main loss is at the outlet/sample collection: tangential filtration design in drawing stage.
  • Contacted EC Shaw about rubber stamp mold for new design for evaporation.
  • Set up COMSOL and StarCD, look for governing equations for simulation of evaporation.
  • Committee meeting setup


† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan)

  • Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between. Deliver CAD drawings for these for photomasks by Friday 10/16, functional chips to be delivered by 10/26 [Hussam/Jessie]
  • Silica free SPE, and SPE free channel experiment to be repeated. Control was old didn't take pressure, report back on 10/15.
  • Load DNA in a straw of 50ul SPE, quick test of hypothesis more SPE = more DNA, report back 10/16.


† PCR - CMI (Lead: Qingqing)

  • QQ will work on the initial integration steps of SPE + RT (resevoir)+ PCR.
  • QQ: Paper back to QQ 9/29.
  • CMK: PCR 1 draft. Analytical Chem. MCK is running more simulations.
  • made 40 cycle chip.
  • Success with 30 cycles, Qiagen Kit. Look at annealing temperature as per Alexis.
  • Integration chip for Qiagen RT-PCR one tube reaction. Control sample first, patient sample second.


† HDA (Lead: Jaephil, Team:Sonali)

  • Start planning R01 for Submission on 2/5/10. MM, JD, CMK.
  • Paper submitted 10/6.


‡C. diff Project (Cathie, Sonali, Satish Singh, His post doc )

  • Sonali to train Lisa on SPE.
  • QQ to run test PCR on chip with genomic DNA and Toxin B primers.


‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing)

  • Meeting 9am Monday 9/14 (every other week)


‡ Agilent Automated Sample Preparation (Lead: Alex)

  • With Straws in Parallel at this point. Speeding up the assay.
  • Waiting for the machine.
  • Alex drafting abstracts.


‡ COBRA (Lead: Jaephil, Team: Cathie, Jane for virus only, PHO folks)

  • Metal piece modification for the integration - Parts distored and is begin fixed.
  • Virus concentration (dialyzed against PBS, no SERS signal, dialyze against water now).
  • paper 1: Evap with Sol Gel substrate. Not integrated. MSSA, E coli.


‡ Biointerfaces group (Lead: MinCheol, Team: Cathie, MCK, Wong and Meller folks)

  • New experiments happening now.


‡ CIMIT- Sepsis (Lead:Cathie, Team:TBA)

  • IRB approved.
  • Cathie submitted the companion BU IRB form for exemption. Revised, still still waiting.


‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME), Suma)

  • Phone meeting with PATH this week.
  • Training on straw making concluded.



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