Klapperich Lab:Notebook/Lab Meeting Notes/2009/10/13: Difference between revisions
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† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | † SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | ||
* RNA extraction troubleshooting. Do deinhibition of monolith components. Test those with the Ambion RNAse kit. Jessie this week. <br> | * RNA extraction troubleshooting. Do deinhibition of monolith components. Test those with the Ambion RNAse kit. Jessie this week. <br> | ||
* | * Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between. Deliver CAD drawings for these for photomasks by Friday 10/16, functional chips to be delivered by 10/26 [hussam/Jessie] | ||
*Silica free SPE, and SPE free channel experiment to be repeated. Control was old didn't take pressure, report back on 10/15. | |||
*Silica free SPE, and SPE free channel experiment | *Experiment with Alex to load DNA in a straw of 50ul SPE, quick test of hypothesis more SPE = more DNA. | ||
* Jessie worked with plasmid. Good std. curve. Not good predictor of the real sample amount. J will redo math with Sonali and Hussam to check.<br> | * Jessie worked with plasmid. Good std. curve. Not good predictor of the real sample amount. J will redo math with Sonali and Hussam to check.<br> | ||
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Revision as of 10:05, 13 October 2009
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13 October 2009 Lab Meeting
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