Klapperich Lab:Notebook/Lab Meeting Notes/2009/10/20: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Qingqing Cao (talk | contribs) No edit summary |
Cmklapperich (talk | contribs) No edit summary |
||
Line 21: | Line 21: | ||
<br> | <br> | ||
† Sample Concentration (Lead: Jane, Team: Jaephil) <br> | † Sample Concentration (Lead: Jane, Team: Jaephil) <br> | ||
* MDCK Cells are not propagating. Try troubleshooting efforts. Sonali will do on Monday. <br> | |||
* Cassidy needs to see plaque assay again - so when cells are up, she needs training. <br> | |||
* Up to high 10^7 for positive control. Silver substrate no signal. <br> | * Up to high 10^7 for positive control. Silver substrate no signal. <br> | ||
* Jane working on the cell lysate control | * Jane working on the cell lysate control. <br> | ||
* Main loss is at the outlet/sample collection: tangential filtration design in drawing stage. <br> | * Main loss is at the outlet/sample collection: tangential filtration design in drawing stage. <br> | ||
* Contacted EC Shaw about rubber stamp mold for new design for evaporation. <br> | * Contacted EC Shaw about rubber stamp mold for new design for evaporation. <br> | ||
Line 32: | Line 33: | ||
† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | † SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | ||
* Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between.Send out masks by 10/21, functional chips to be delivered by 10/26. [Update]<i> functional chips ready by 10/21-22, Experimental results of channels to be delivered on 10/27 </i> [Hussam/Jessie]<br> | * Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between.Send out masks by 10/21, functional chips to be delivered by 10/26. [Update]<i> functional chips ready by 10/21-22, Experimental results of channels to be delivered on 10/27 </i> [Hussam/Jessie]<br> | ||
* STD recipe, STD silica beads, just A,B, and C. <br> | |||
* controls for these: empties, non-silica monolith and full silica monolith, std. recipe. <br> | |||
* Load DNA in a straw of 5,50,100ul SPE, quick test of hypothesis: more SPE = more DNA, <i>To be repeated with Alex and Mark </i> <br> | * Load DNA in a straw of 5,50,100ul SPE, quick test of hypothesis: more SPE = more DNA, <i>To be repeated with Alex and Mark </i> <br> | ||
<br> | <br> |
Revision as of 12:09, 20 October 2009
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
20 October 2009 Lab Meeting
† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan)
* test liquids to fill the empty channel(Mineral oil,Water) * Deep reservoir design - deformation during bonding - fabrication difficulty - Fluidic control difficulty * third generation design to solve these problem - the mold and the chip has been made, and will be tested this week.
- ATCC DNA and Primer pairs for both toxin A and B has been ordered. - real-time PCR has been done on ABI, waiting for the result.
|