Klapperich Lab:Notebook/Lab Meeting Notes/2009/10/20: Difference between revisions
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'''‡ Announcements'''<br> | '''‡ Announcements'''<br> | ||
* <br> | * Oakridge deadline is 1 Feb 2010. <br> | ||
* '''PAPERS Drafted before MicroTAS''' <br> | * '''PAPERS Drafted before MicroTAS''' <br> | ||
* '''Posters for MicroTAS'''<br> | * '''Posters for MicroTAS'''<br> | ||
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† Sample Concentration (Lead: Jane, Team: Jaephil) <br> | † Sample Concentration (Lead: Jane, Team: Jaephil) <br> | ||
* MDCK Cells are not propagating. Try troubleshooting efforts. Sonali will do on Monday. <br> | |||
* Cassidy needs to see plaque assay again - so when cells are up, she needs training. <br> | |||
* Up to high 10^7 for positive control. Silver substrate no signal. <br> | * Up to high 10^7 for positive control. Silver substrate no signal. <br> | ||
* Jane working on the cell lysate control | * Jane working on the cell lysate control. <br> | ||
* Main loss is at the outlet/sample collection: tangential filtration design in drawing stage. <br> | * Main loss is at the outlet/sample collection: tangential filtration design in drawing stage. <br> | ||
* Contacted EC Shaw about rubber stamp mold for new design for evaporation. <br> | * Contacted EC Shaw about rubber stamp mold for new design for evaporation. <br> | ||
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† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | † SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | ||
* Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between.Send out masks by 10/21, functional chips to be delivered by 10/26. [Update]<i> functional chips ready by 10/21-22, Experimental results of channels to be delivered on 10/27 </i> [Hussam/Jessie]<br> | * Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between.Send out masks by 10/21, functional chips to be delivered by 10/26. [Update]<i> functional chips ready by 10/21-22, Experimental results of channels to be delivered on 10/27 </i> [Hussam/Jessie]<br> | ||
* STD recipe, STD silica beads, just A,B, and C. <br> | |||
* controls for these: empties, non-silica monolith and full silica monolith, std. recipe. <br> | |||
* Load DNA in a straw of 5,50,100ul SPE, quick test of hypothesis: more SPE = more DNA, <i>To be repeated with Alex and Mark </i> <br> | * Load DNA in a straw of 5,50,100ul SPE, quick test of hypothesis: more SPE = more DNA, <i>To be repeated with Alex and Mark </i> <br> | ||
*'' Look into modifying Alex's pneumatic setup for the PATH guys. Instead of building the straw array over here NOTE: Sean'' <br> | |||
*PLanning net silica per channel experiments. with 700nm, 3x possible. Deliver around 11/5/09.<br> | |||
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* New virus prep (JC, 6/11/09), getting Ct's of 27 for 1:16 dilution and 28 for 1:64 dilution. Use more diluted sample for future optimization experiments?<br> | * New virus prep (JC, 6/11/09), getting Ct's of 27 for 1:16 dilution and 28 for 1:64 dilution. Use more diluted sample for future optimization experiments? Channels were 700nm silica/1X. pfu/ml is 10^6. <br> | ||
* Tried 2 more chips with 3X 0.15um Silica. SPE was coming out even at 2ml/hr flow rate.<br> | * Tried 2 more chips with 3X 0.15um Silica. Chips are breaking b/c of no crosslinking. SPE was coming out even at 2ml/hr flow rate.<br> | ||
* Running no silica and no SPE chips with virus.<br> | * Running no silica and no SPE chips with virus. (JC/RNA)<br> | ||
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† PCR - CMI (Lead: Qingqing) <br> | † PCR - CMI (Lead: Qingqing) <br> | ||
* QQ will work on the initial integration steps of SPE + RT ( | * QQ will work on the initial integration steps of SPE + RT (reservoir)+ PCR. <br> | ||
* test liquids to fill the empty channel(Mineral oil,Water) | |||
* Deep reservoir design | |||
- deformation during bonding | |||
- fabrication difficulty | |||
- Fluidic control difficulty | |||
* third generation design to solve these problem | |||
- the mold and the chip has been made, and will be tested this week. | |||
* PCR of C.Difficile DNA | |||
- ATCC DNA and Primer pairs for both toxin A and B has been ordered. | |||
- real-time PCR has been done on ABI, waiting for the result. | |||
* PCR2 Paper formatted for LOAC this week. <br> | * PCR2 Paper formatted for LOAC this week. <br> | ||
* CMK: PCR 1 draft. Analytical Chem. MCK is running more simulations. <br> | * CMK: PCR 1 draft. Analytical Chem. MCK is running more simulations. <br> | ||
Revision as of 12:59, 20 October 2009
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20 October 2009 Lab Meeting
† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan)
* test liquids to fill the empty channel(Mineral oil,Water) * Deep reservoir design - deformation during bonding - fabrication difficulty - Fluidic control difficulty * third generation design to solve these problem - the mold and the chip has been made, and will be tested this week.
- ATCC DNA and Primer pairs for both toxin A and B has been ordered. - real-time PCR has been done on ABI, waiting for the result.
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