Klapperich Lab:Notebook/Lab Meeting Notes/2009/10/20: Difference between revisions
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'''‡ Announcements'''<br> | '''‡ Announcements'''<br> | ||
* <br> | * Oakridge deadline is 1 Feb 2010. <br> | ||
* '''PAPERS Drafted before MicroTAS''' <br> | * '''PAPERS Drafted before MicroTAS''' <br> | ||
* '''Posters for MicroTAS'''<br> | * '''Posters for MicroTAS'''<br> | ||
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*PLanning net silica per channel experiments. with 700nm, 3x possible. Deliver around 11/5/09.<br> | *PLanning net silica per channel experiments. with 700nm, 3x possible. Deliver around 11/5/09.<br> | ||
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* New virus prep (JC, 6/11/09), getting Ct's of 27 for 1:16 dilution and 28 for 1:64 dilution. Use more diluted sample for future optimization experiments?<br> | * New virus prep (JC, 6/11/09), getting Ct's of 27 for 1:16 dilution and 28 for 1:64 dilution. Use more diluted sample for future optimization experiments? Channels were 700nm silica/1X. pfu/ml is 10^6. <br> | ||
* Tried 2 more chips with 3X 0.15um Silica. SPE was coming out even at 2ml/hr flow rate.<br> | * Tried 2 more chips with 3X 0.15um Silica. Chips are breaking b/c of no crosslinking. SPE was coming out even at 2ml/hr flow rate.<br> | ||
* Running no silica and no SPE chips with virus.<br> | * Running no silica and no SPE chips with virus. (JC/RNA)<br> | ||
<br> | <br> | ||
† PCR - CMI (Lead: Qingqing) <br> | † PCR - CMI (Lead: Qingqing) <br> |
Revision as of 12:59, 20 October 2009
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20 October 2009 Lab Meeting
† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan)
* test liquids to fill the empty channel(Mineral oil,Water) * Deep reservoir design - deformation during bonding - fabrication difficulty - Fluidic control difficulty * third generation design to solve these problem - the mold and the chip has been made, and will be tested this week.
- ATCC DNA and Primer pairs for both toxin A and B has been ordered. - real-time PCR has been done on ABI, waiting for the result.
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