Klapperich Lab:Notebook/Lab Meeting Notes/2009/10/20: Difference between revisions
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† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | † SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | ||
* Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between. | * Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between. functional chips to be delivered by 10/21-22, Experimental results of channel to be delivered 10/27 [Hussam/Jessie] | ||
* Load DNA in a straw of 5,50,100ul SPE, quick test of hypothesis: more SPE = more DNA, <i>To be repeated with Alex and Mark </i> <br> | |||
* Load DNA in a straw of | |||
* New virus prep (JC, 6/11/09), getting Ct's of 27 for 1:16 dilution and 28 for 1:64 dilution. Use more diluted sample for future optimization experiments?<br> | * New virus prep (JC, 6/11/09), getting Ct's of 27 for 1:16 dilution and 28 for 1:64 dilution. Use more diluted sample for future optimization experiments?<br> | ||
* Tried 2 more chips with 3X 0.15um Silica. SPE was coming out even at 2ml/hr flow rate.<br> | * Tried 2 more chips with 3X 0.15um Silica. SPE was coming out even at 2ml/hr flow rate.<br> |
Revision as of 09:54, 20 October 2009
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20 October 2009 Lab Meeting
† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan)
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