Klapperich Lab:Notebook/Lab Meeting Notes/2009/10/27: Difference between revisions
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† PCR - CMI (Lead: Qingqing) <br> | † PCR - CMI (Lead: Qingqing) <br> | ||
* QQ will work on the initial integration steps of SPE + RT (reservoir)+ PCR. <br> | * QQ will work on the initial integration steps of SPE + RT (reservoir)+ PCR. <br> | ||
* New design has been tested | |||
- It solved some problems, however bubbles were still created at the high-temperature section at slow flow rate. And they grow up to big bubbles and did not move along the channel,which affect the moving of reagent. | |||
- some new method will be tested this week to solve this problem. | |||
* PCR of C.Difficile DNA | * PCR of C.Difficile DNA | ||
- | - primer for toxin A. | ||
- real-time | a.300nM did not amplify on real-time machine.nothing detected by bio-analyzer | ||
b.700nM had a ct value of 27.A small peak (0.63ng/ul) was detected on bio-analyzer | |||
- DNA template dilution experiments has been done. | |||
- Primer for toxin B did not work on real-time machine. No result was detected by bio-analyzer. | |||
* PCR2 Paper formatted for LOAC this week. <br> | * PCR2 Paper formatted for LOAC this week. <br> | ||
need to get back from Cathie | |||
* CMK: PCR 1 draft. Analytical Chem. MCK is running more simulations. <br> | * CMK: PCR 1 draft. Analytical Chem. MCK is running more simulations. <br> | ||
<br> | <br> |
Revision as of 08:23, 27 October 2009
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27 October 2009 Lab Meeting
† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan)
* New design has been tested - It solved some problems, however bubbles were still created at the high-temperature section at slow flow rate. And they grow up to big bubbles and did not move along the channel,which affect the moving of reagent. - some new method will be tested this week to solve this problem.
- primer for toxin A. a.300nM did not amplify on real-time machine.nothing detected by bio-analyzer b.700nM had a ct value of 27.A small peak (0.63ng/ul) was detected on bio-analyzer - DNA template dilution experiments has been done. - Primer for toxin B did not work on real-time machine. No result was detected by bio-analyzer.
need to get back from Cathie
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