Klapperich Lab:Notebook/Lab Meeting Notes/2009/10/27: Difference between revisions
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† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | † SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | ||
* | *Video with two color dyes and [red(primer) then blue, green, water+tween] | ||
*repeat experiment reported on 10/27 with 700nm Silica 3X. | |||
* controls for these: empties, non-silica monolith and full silica monolith, std. recipe. <br> | * controls for these: empties, non-silica monolith and full silica monolith, std. recipe. <br> | ||
* Load DNA in a straw of 5,50,100ul SPE, quick test of hypothesis: more SPE = more DNA, <i>To be repeated with Alex and Mark </i> <br> | * Load DNA in a straw of 5,50,100ul SPE, quick test of hypothesis: more SPE = more DNA, <i>To be repeated with Alex and Mark </i> <br> | ||
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* QQ will work on the initial integration steps of SPE + RT (reservoir)+ PCR. <br> | * QQ will work on the initial integration steps of SPE + RT (reservoir)+ PCR. <br> | ||
* new design has been tested <br> | * new design has been tested <br> | ||
It solved some problems, however bubbles were still created at the high-temperature section at slow flow rate. And they grow up to big bubbles | It solved some problems, however bubbles were still created at the <br> high-temperature section at slow flow rate. And they grow up to big <br> bubbles and did not move along the channel, which affect the moving of <br> reagent. | ||
and did not move along the channel, which affect the moving of reagent. | - some new method will be tested this week to solve this problem. | ||
* PCR of C.Difficile DNA | * PCR of C.Difficile DNA | ||
- primer for toxin A. | - primer for toxin A. | ||
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b.700nM had a ct value of 27.A small peak (0.63ng/ul) was detected on bio-analyzer | b.700nM had a ct value of 27.A small peak (0.63ng/ul) was detected on bio-analyzer | ||
- DNA template dilution experiments has been done. | - DNA template dilution experiments has been done. | ||
- Primer for toxin B did not work on real-time machine. No result was detected by bio-analyzer. | - Primer for toxin B did not work on real-time machine. No result was<br> detected by bio-analyzer. | ||
* PCR2 Paper formatted for LOAC this week. <br> | * PCR2 Paper formatted for LOAC this week. <br> |
Revision as of 12:40, 27 October 2009
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27 October 2009 Lab Meeting
† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan)
* new design has been tested
- primer for toxin A. a.300nM did not amplify on real-time machine.nothing was detected by bio-analyzer b.700nM had a ct value of 27.A small peak (0.63ng/ul) was detected on bio-analyzer - DNA template dilution experiments has been done. - Primer for toxin B did not work on real-time machine. No result was
need to get back from Cathie
‡ COBRA (Lead: Jaephil, Team: Cathie, Jane for virus only, PHO folks)
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