Klapperich Lab:Notebook/Lab Meeting Notes/2009/11/10: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Qingqing Cao (talk | contribs) No edit summary |
|||
Line 100: | Line 100: | ||
'''‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME), Suma)''' <br> | '''‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME), Suma)''' <br> | ||
* Phone meeting with PATH this week. 10/13. <br> | * Phone meeting with PATH this week. 10/13. <br> | ||
* Frank | * Senior Project Proposals due in less than two weeks! (23 Nov.) <br> | ||
* | * Frank: 1-week unstabilized sample PCRed (increased degradation with higher storage temps as expected; eluting 2-week sample on Wed. <br> | ||
* Frank: PVA to be ordered for home-made polymer storage solution; begin solubility tests upon arrival. <br> | |||
* Sean is currently working on designing a straw-holding piece for the new fixture in 709. <br> | * Sean is currently working on designing a straw-holding piece for the new fixture in 709. <br> | ||
* Sean met with Alex for an integration of straws on black machine. They sketched a design. Sean will make a professional drawing and create a required parts list. Alex will supervise the design. Before handing in the job to the shop floor, the design will be presented to Cathie. <br> | * Sean met with Alex for an integration of straws on black machine. They sketched a design. Sean will make a professional drawing and create a required parts list. Alex will supervise the design. Before handing in the job to the shop floor, the design will be presented to Cathie. <br> |
Revision as of 16:16, 9 November 2009
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
10 November 2009 Lab Meeting
† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan)
- third design was tested with water,big bubbles observed, but the water still could be collected at the outlet. - third design was tested with PCR reagent(Flu assay). On-chip PCR does not work. - the same experiment was repeated with the reduced hot-start time(five minutes). the on-chip PCR still does not work. * New channel has been designed. - molds(SU-8,PDMS,Epox)has been made. - chip has been made and tested with water. Both thermal and fluidic control are fine. it will be tested with PCR reagent this week.
- primer for toxin A. - on chip PCR with the optimized primer and template concentration does not work. - MgCl2 concentration was optimized to improve the PCR efficiency. - on chip PCR with the optimized MgCl2 concentration does not work. - Primer for toxin B. New primer sequence from Lisa has been ordered, will be tested when they come.
need to get back from Cathie
‡ COBRA (Lead: Jaephil, Team: Cathie, Jane for virus only, PHO folks)
|