Klapperich Lab:Notebook/Lab Meeting Notes/2010/02/25: Difference between revisions
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=25 February 2010 Lab Meeting= | =25 February 2010 Lab Meeting= | ||
Alex Presentation. | |||
'''‡ Announcements'''<br> | '''‡ Announcements'''<br> | ||
'''‡ Flu R01:Integration''' <br> | '''‡ Flu R01:Integration''' <br> | ||
* meetings every other M 1-3pm. 705. <br> | * meetings every other M 1-3pm. 705. <br> | ||
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† HDA (Lead: Jaephil, Team:Sonali)<br> | † HDA (Lead: Jaephil, Team:Sonali)<br> | ||
* Start planning R01 for Submission on '''6/5/10.''' MM, JD, CMK. <br> | * Start planning R01 for Submission on '''6/5/10.''' MM, JD, CMK. <br> | ||
* HDA mix in dried form - | * HDA mix in dried form - Expecting it from BH. <br> | ||
* HDA in tube works up to 69C. Negative at 70C. 65C is best. No temps below 65C tested. Confirms manufacturer papers and protocol (MM) <br> | * HDA in tube works up to 69C. Negative at 70C. 65C is best. No temps below 65C tested. Confirms manufacturer papers and protocol (MM) <br> | ||
* LOD tests completed by MM on chip/in cup for 10ng, 0.3ng, 0.03ng, 0.003ng, 0.0003ng. Amplicon present down to 3pg, though very faint. Negative at 0.3pg <br> | * LOD tests completed by MM on chip/in cup for 10ng, 0.3ng, 0.03ng, 0.003ng, 0.0003ng. Amplicon present down to 3pg, though very faint. Negative at 0.3pg <br> | ||
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* testing real patient sample | * testing real patient sample | ||
* PCR1 | * PCR1 will combine with PCR2 if PCR2 comes back for revisions. (CMK)<br> | ||
<br> | <br> | ||
'''‡C. diff Project''' (Cathie, Sonali, Satish Singh, Lisa J., His post doc )<br> | '''‡C. diff Project''' (Cathie, Sonali, Satish Singh, Lisa J., His post doc )<br> | ||
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<br> | <br> | ||
'''‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing)''' | '''‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing)''' | ||
* New meeting time, T, 9:30 am every other week. <br> | * New meeting time, T, 9:30 am every other week. MEETINGS CANCELLED THIS WEEK. <br> | ||
* Will be done by next Tuesday - PCR with 3 different kits of same serial dilution RNA sample (Invitrogen - SuperscriptIII Platinum, Ambion - AgPath-ID, Qiagen - OneStep RT-PCR Kit)<br> | * Will be done by next Tuesday - PCR with 3 different kits of same serial dilution RNA sample (Invitrogen - SuperscriptIII Platinum, Ambion - AgPath-ID, Qiagen - OneStep RT-PCR Kit)<br> | ||
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Revision as of 14:15, 24 February 2010
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25 February 2010 Lab MeetingAlex Presentation. ‡ Announcements ‡ Flu R01:Integration
† HDA (Lead: Jaephil, Team:Sonali)
† Sample Concentration (Lead: Jane, Team: Jaephil)
† SPE Column Optimization for DNA/RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan)
† integrated chip for flu(SPE+RT+PCR) (Lead: Qingqing)
† PCR of C.Difficile DNA (Qingqing)
* wrong size products(55bp). * no designed size product.
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