Klapperich Lab:Notebook/Lab Meeting Notes/2010/02/25: Difference between revisions
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† HDA (Lead: Jaephil, Team:Sonali)<br> | † HDA (Lead: Jaephil, Team:Sonali)<br> | ||
* Patient Samples - dilution experiments (MM/SH/JD) <br> | |||
* Start planning R01 for Submission on '''6/5/10.''' MM, JD, CMK. <br> | * Start planning R01 for Submission on '''6/5/10.''' MM, JD, CMK. <br> | ||
* HDA mix in dried form - Expecting it from BH. <br> | * HDA mix in dried form - Expecting it from BH. <br> | ||
* | * warm start on the way <br> | ||
* HDA negative water reactions all have primer dimers/non-specific band at 70bp for c diff HDA (MM)<br> | * HDA negative water reactions all have primer dimers/non-specific band at 70bp for c diff HDA (MM)<br> | ||
* 2nd HDA design - "microSPE" + "Flu primer" + "HDA in a cup" + "Detection kit from Biohelix"<br> | * 2nd HDA design - "microSPE" + "Flu primer" + "HDA in a cup" + "Detection kit from Biohelix"<br> | ||
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† Sample Concentration (Lead: Jane, Team: Jaephil) <br> | † Sample Concentration (Lead: Jane, Team: Jaephil) <br> | ||
* Air resistance in the air channel is too large. <br> | * Air resistance in the air channel is too large. <br> | ||
** New branched "membrane" chip design #1 in lithography stage. <br> | ** New branched "membrane" chip design #1 in lithography stage. <br> | ||
** New "tube" chip design in drawing stage. Obtained more porous PTFE tubing samples <br> | ** New "tube" chip design in drawing stage. Obtained more porous PTFE tubing samples <br> | ||
* Samples expected from Fauchet group. <br> | |||
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† SPE Column Optimization for DNA/RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | † SPE Column Optimization for DNA/RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | ||
* Sonali put out a new RNA protocol (correct SPE batter, homemade buffers, less ethanol washing, RNA Secure) before break. <br> | * Sonali put out a new RNA protocol (correct SPE batter, homemade buffers, less ethanol washing, RNA Secure) before break. <br> | ||
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* Sonali completed 5 total patients (BUMC and BIDMC) extracted and RT-PCR'd successfully using new RNA protocol and small SPE channel chips. All samples selected based on Qiagen kit extractions having low Cts at 15-20 cycles <br> | * Sonali completed 5 total patients (BUMC and BIDMC) extracted and RT-PCR'd successfully using new RNA protocol and small SPE channel chips. All samples selected based on Qiagen kit extractions having low Cts at 15-20 cycles <br> | ||
* Sonali gave Qingqing 3 of these patient RNAs extracted on chip SPE for testing on-chip RT-PCR with SuperScript vs Qiagen one-Step kit. <br> | * Sonali gave Qingqing 3 of these patient RNAs extracted on chip SPE for testing on-chip RT-PCR with SuperScript vs Qiagen one-Step kit. <br> | ||
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† | † Integrated chip for flu(SPE+RT+PCR) (Lead: Qingqing) <br> | ||
* integrated chip works with ATCC flu virus <br> | * integrated chip works with ATCC flu virus <br> | ||
* working on improving the efficiency | * working on improving the efficiency | ||
* testing real patient sample | * testing real patient sample <br> | ||
* PCR1 will combine with PCR2 if PCR2 comes back for revisions. (CMK)<br> | * PCR1 will combine with PCR2 if PCR2 comes back for revisions. (CMK)<br> |
Revision as of 13:01, 25 February 2010
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25 February 2010 Lab MeetingAlex Presentation. ‡ Announcements ‡ Flu R01:Integration
† HDA (Lead: Jaephil, Team:Sonali)
† PCR of C.Difficile DNA (Qingqing)
* wrong size products(55bp). * no designed size product.
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