Klapperich Lab:Notebook/Lab Meeting Notes/2010/03/18: Difference between revisions
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† SPE Column Optimization for DNA/RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | † SPE Column Optimization for DNA/RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | ||
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* http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F5155018 <br> | |||
* (In progress) Jessie will run chips with serial virus dilutions from 1:1 to 1:1,000,000 to re-do previous dilution experimnent with WHO SYBR green assay on chip.<br> | * (In progress) Jessie will run chips with serial virus dilutions from 1:1 to 1:1,000,000 to re-do previous dilution experimnent with WHO SYBR green assay on chip.<br> | ||
* Less input sample (same concentration). | * Less input sample (same concentration, 37.5uL sample vs 100uL sample). From raw data (from 30uL extracted RNA), less sample gives significantly(p-value = 0.0005) less copies. If normalized to per mL of sample input, copies/mL are comparable between two sample inputs (p-value = 0.5094).(JC) <br> | ||
* Plaque assay on patient samples for QQ's paper. <br> | * Plaque assay on patient samples for QQ's paper. <br> | ||
* Casein in human serum experiments ongoing. (HM) <br> | * Casein in human serum experiments ongoing. (HM) <br> | ||
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* Frank: One week data up 3/5/10. Starting Friday will do saturation/capacity experiment. Longer-term time points with current protocol will be run this weekend.<br> | * Frank: One week data up 3/5/10. Starting Friday will do saturation/capacity experiment. Longer-term time points with current protocol will be run this weekend.<br> | ||
* Sean: Started making straws for the new experiments we talked about (DNA weights of 1ng, 10ng, and 100ng through 700nm at SPE 100 monolith at 0-day time point). <br> | * Sean: Started making straws for the new experiments we talked about (DNA weights of 1ng, 10ng, and 100ng through 700nm at SPE 100 monolith at 0-day time point). <br> | ||
* Mark: as of 3/17/10 up to 10% recovery from 10 ng input sample with water elution. Also up to 13% recovery with 100ng/straw. Loaded two sample groups for multiple temperatures for 1-week and 2-week/1-month time points.<br> | * Mark: as of 3/17/10 up to 10% recovery from 10 ng input sample with water elution. Also up to 13% recovery with 100ng/straw. Loaded two sample groups for multiple temperatures for 1-week and 2-week/1-month time points. 1-week trial completed as of 3/24. Results peak at 20% (-70C) and decrease with temperature. Next to test straws with Qiagen buffer.<br> | ||
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'''‡ IIH Senior Project.'''<br> | '''‡ IIH Senior Project.'''<br> |
Revision as of 07:24, 25 March 2010
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18 March 2010 Lab MeetingJason Keller Presentation. ‡ Announcements ‡ Flu R01:Integration
† HDA (Lead: Jaephil, Team:Sonali)
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