Klapperich Lab:Notebook/Lab Meeting Notes/2010/04/15: Difference between revisions
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'''‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME))''' <br> | '''‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME))''' <br> | ||
* Phone call with PATH 3/22. Frank has class 2-4 but will come ASAP.<br> | * Phone call with PATH 3/22. Frank has class 2-4 but will come ASAP.<br> | ||
* Frank: | * Frank: Two- and three-week storage experiments completed. Four-week to be done next Wed. Longer-term (2, 3, 4-month) in storage. +/- carrier RNA not yet completed. Currently writing up final report.<br> | ||
* Sean: First set of straws is finished (700 nm at varying SPE values, 140 SPE down to 0 SPE in increments fo 20 SPE). Samples to be run tomorrow morning (4.2.10). NB: these straws have only 16ul of monolith in them. This could possibly affect the outcome, but we anticipate that if all straws for all silica sizes are made with the same 16ul amount, the results will be just as reliable as if ~50ul had been used. <br> | * Sean: First set of straws is finished (700 nm at varying SPE values, 140 SPE down to 0 SPE in increments fo 20 SPE). Samples to be run tomorrow morning (4.2.10). NB: these straws have only 16ul of monolith in them. This could possibly affect the outcome, but we anticipate that if all straws for all silica sizes are made with the same 16ul amount, the results will be just as reliable as if ~50ul had been used. <br> | ||
* Mark: as of 3/17/10 up to 10% recovery from 10 ng input sample with water elution. Also up to 13% recovery with 100ng/straw. Loaded two sample groups for multiple temperatures for 1-week and 2-week/1-month time points. 1-week trial completed as of 3/24. Results peak at 20% (-70C) and decrease with temperature. Next to test straws with Qiagen buffer.<br> | * Mark: as of 3/17/10 up to 10% recovery from 10 ng input sample with water elution. Also up to 13% recovery with 100ng/straw. Loaded two sample groups for multiple temperatures for 1-week and 2-week/1-month time points. 1-week trial completed as of 3/24. Results peak at 20% (-70C) and decrease with temperature. Next to test straws with Qiagen buffer.<br> |
Revision as of 08:24, 15 April 2010
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15 April 2010 Lab MeetingJake Presentation. ‡ Announcements ‡ Flu R01:Integration
† HDA (Lead: Jaephil, Team:Sonali)
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