Klapperich Lab:Notebook/Lab Meeting Notes/2010/04/22: Difference between revisions
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'''‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME))''' <br> | '''‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME))''' <br> | ||
*.<br> | *.<br> | ||
* Frank: | * Frank: Four-week storage results sent to PATH group. +/- carrier RNA results sent as well -- see increased ng amount extracted withOUT RNA, but not statistically significant (~60 vs. 40ng). Longer-term (2, 3, 4-month) in storage. Currently writing up final report for senior project. After classes end, will be compiling documents/organizing supplies for whoever is taking over the project.<br> | ||
* Sean: First set of data is in (for the 700 nm silica straws). Today (4/15) I will finish the straws for the 150 nm silica and the SPE batters for the 14 nm silica. Tomorrow I plan on running and PCR'ing samples for the 150 nm silica straws as well as making the straws for the 14 nm silica. On Saturday I will run and PCR samples for the last set of straws. <br> | * Sean: First set of data is in (for the 700 nm silica straws). Today (4/15) I will finish the straws for the 150 nm silica and the SPE batters for the 14 nm silica. Tomorrow I plan on running and PCR'ing samples for the 150 nm silica straws as well as making the straws for the 14 nm silica. On Saturday I will run and PCR samples for the last set of straws. <br> | ||
* Mark: as of 4/15/10 up to 30% recovery from 10 ng input sample with water elution. Also up to 13% recovery with 100ng/straw. 1-week trial completed as of 3/24. Results peak at 20% (-70C) and decrease with temperature. 2-month and 1-month trials complete, resulting in further degradation than in the 1-week trial. 4- and 6-month trials still in storage.<br> | * Mark: as of 4/15/10 up to 30% recovery from 10 ng input sample with water elution. Also up to 13% recovery with 100ng/straw. 1-week trial completed as of 3/24. Results peak at 20% (-70C) and decrease with temperature. 2-month and 1-month trials complete, resulting in further degradation than in the 1-week trial. 4- and 6-month trials still in storage.<br> |
Revision as of 09:04, 22 April 2010
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22 April 2010 Lab Meeting‡ Announcements ‡ Flu R01:Integration
† HDA (Lead: Jaephil, Team:Sonali)
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