Klapperich Lab:Notebook/Lab Meeting Notes/2010/04/29: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2010/04/29 Entry for Klapperich_Lab:Notebook/Lab_Meeting_Notes)
 
No edit summary
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
=29 April 2010 Lab Meeting=


* Senior Project Practice presentations. <br>
'''‡ Announcements'''<br>
* May 6 Lab meeting cancelled <br>
* Email me times that you CANNOT make for lab meetings starting May 13th. <br>
'''‡ Flu R01:Integration''' <br>
* meetings every other M 1-3pm. 725.  <br>
** SEQUENCING WORK<br>
* for PCR just do more reactions, pool and etoh precipitate. <br>
** For HDA, we need to explore cloning. Waiting for the competent cells. <br>
** dxs PCR primers worked for HDA in tube. Still no positive results on chip. <br>
* Check other methods of heating. <br>
† HDA (Lead:Sonali)<br>
* IBC Approval or 720 to work with real C.diff samples. <br>
* Patient Samples - dilution experiments (MM/SH) <br>
* Start planning R01 for Submission on '''10/5/10.''' MM, CMK. <br>
* HDA mix in dried form - Expecting it from BH -- ON HOLD. <br>
* <br>
* QQ preliminary chip work <br>
* Sequence all output <br>
* 2nd HDA design - "microSPE" + "Flu primer" + "HDA in a cup" + "Detection kit from Biohelix"<br>
<br>
† Sample Concentration (Lead: Jane, Team: Jaephil) <br>
* Straight channel adhesion test PCR result expected. From 4/6 result, PDMS 4mm wide channel doesn't show decrease in output RNA over time <br>
* Straight 1mm and 4mm PDMS-Done <br>
* Try collection vs dilutions, collection vs time, need binding constant to quantify results. <br>
* <br>
<br>
† SPE Column Optimization for DNA/RNA. (Lead: ) <br>
* (Almost done) Jessie will run chips with serial virus dilutions from 1:1 to 1:1,000,000 to re-do previous dilution experimnent with WHO SYBR green assay on chip.<br>
* <br>
<br>
† Integrated chip for flu(SPE+RT+PCR) (Lead: Qingqing) <br>
* Real copy number of previous human samples. So QQ is working with the higher concentration samples. Range is 10^6-10^8 of copy number/ml.  <br>
* REVISIT the % recovery number. <br>
* QQ tried: more enzyme, Tween 20, BSA amounts. <br>
* integrated flu chip paper has been send to cathie, wait for revision.
<br>
* PCR1 will combine with PCR2, waitting for revision (CMK)<br>
<br>
'''‡C. diff Project''' (Cathie, Sonali, Satish Singh, Lisa J., His post doc )<br>
* Discuss Project Goals Today <br>
<br>
'''‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing)'''
* New meeting time, T, 9:30 am every other week. MEETINGS CANCELLED NEXT WEEK (4/20). <br>
<br>
'''‡ Agilent Automated Sample Preparation (Lead: Alex)'''<br>
* Paper on HotDog - with CMK/ASB  <br>
<br>
'''‡ COBRA (Lead: Jaephil, Team: Cathie, Jane for virus only, PHO folks)<br>'''
* Evap paper - Back to Jane 4/29. <br>
<br>
'''‡ Biointerfaces group (Lead: MinCheol, Team: Cathie, MCK, Wong and Meller folks)'''<br>
'''* Cathie sent latest draft to MCK.<br>'''
<br>
'''‡ CIMIT- Sepsis (Lead:Cathie, Team:TBA)'''<br>
* Sample collection ongoing. <br>
<br>
'''‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME))''' <br>
*.<br>
* Frank: Four-week storage results sent to PATH group.  +/- carrier RNA results sent as well -- see increased ng amount extracted withOUT RNA, but not statistically significant (~60 vs. 40ng).  Longer-term (2, 3, 4-month) in storage.  Currently writing up final report for senior project.  After classes end, will be compiling documents/organizing supplies for whoever is taking over the project.<br>
* Sean: Data for 700 nm and 150 nm analyzed and it shows that 700 is better overall, and is best in the 60 - 80 SPE range.  Final report almost complete and due tomorrow (4/23).  I will get stuff together and leave my notebook for future research as well. <br>
* Mark: as of 4/15/10 up to 30% recovery from 10 ng input sample with water elution.  Also up to 13% recovery with 100ng/straw.  1-week trial completed as of 3/24. Results peak at 20% (-70C) and decrease with temperature.  2-month and 1-month trials complete, resulting in further degradation than in the 1-week trial. 4- and 6-month trials still in storage.  Final Report draft submitted, due tomorrow.  Presentation next Friday 4/30.  Lab notebook will be updated and ready for future PATH SNAP workers.<br>
<br>
'''‡ IIH Senior Project.'''<br>
* Ordered new HRP substrate. waiting for delivery
* Will run ELISA in chip and image for standard curve.
* finalizing report




Revision as of 08:19, 29 April 2010

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

29 April 2010 Lab Meeting

  • Senior Project Practice presentations.

‡ Announcements

  • May 6 Lab meeting cancelled
  • Email me times that you CANNOT make for lab meetings starting May 13th.

‡ Flu R01:Integration

  • meetings every other M 1-3pm. 725.
    • SEQUENCING WORK
  • for PCR just do more reactions, pool and etoh precipitate.
    • For HDA, we need to explore cloning. Waiting for the competent cells.
    • dxs PCR primers worked for HDA in tube. Still no positive results on chip.
  • Check other methods of heating.

† HDA (Lead:Sonali)

  • IBC Approval or 720 to work with real C.diff samples.
  • Patient Samples - dilution experiments (MM/SH)
  • Start planning R01 for Submission on 10/5/10. MM, CMK.
  • HDA mix in dried form - Expecting it from BH -- ON HOLD.

  • QQ preliminary chip work
  • Sequence all output
  • 2nd HDA design - "microSPE" + "Flu primer" + "HDA in a cup" + "Detection kit from Biohelix"


† Sample Concentration (Lead: Jane, Team: Jaephil)

  • Straight channel adhesion test PCR result expected. From 4/6 result, PDMS 4mm wide channel doesn't show decrease in output RNA over time
  • Straight 1mm and 4mm PDMS-Done
  • Try collection vs dilutions, collection vs time, need binding constant to quantify results.


† SPE Column Optimization for DNA/RNA. (Lead: )

  • (Almost done) Jessie will run chips with serial virus dilutions from 1:1 to 1:1,000,000 to re-do previous dilution experimnent with WHO SYBR green assay on chip.


† Integrated chip for flu(SPE+RT+PCR) (Lead: Qingqing)

  • Real copy number of previous human samples. So QQ is working with the higher concentration samples. Range is 10^6-10^8 of copy number/ml.
  • REVISIT the % recovery number.
  • QQ tried: more enzyme, Tween 20, BSA amounts.
  • integrated flu chip paper has been send to cathie, wait for revision.
  • PCR1 will combine with PCR2, waitting for revision (CMK)


‡C. diff Project (Cathie, Sonali, Satish Singh, Lisa J., His post doc )

  • Discuss Project Goals Today


‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing)

  • New meeting time, T, 9:30 am every other week. MEETINGS CANCELLED NEXT WEEK (4/20).


‡ Agilent Automated Sample Preparation (Lead: Alex)

  • Paper on HotDog - with CMK/ASB


‡ COBRA (Lead: Jaephil, Team: Cathie, Jane for virus only, PHO folks)

  • Evap paper - Back to Jane 4/29.


‡ Biointerfaces group (Lead: MinCheol, Team: Cathie, MCK, Wong and Meller folks)
* Cathie sent latest draft to MCK.
‡ CIMIT- Sepsis (Lead:Cathie, Team:TBA)

  • Sample collection ongoing.


‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME))

  • .
  • Frank: Four-week storage results sent to PATH group. +/- carrier RNA results sent as well -- see increased ng amount extracted withOUT RNA, but not statistically significant (~60 vs. 40ng). Longer-term (2, 3, 4-month) in storage. Currently writing up final report for senior project. After classes end, will be compiling documents/organizing supplies for whoever is taking over the project.
  • Sean: Data for 700 nm and 150 nm analyzed and it shows that 700 is better overall, and is best in the 60 - 80 SPE range. Final report almost complete and due tomorrow (4/23). I will get stuff together and leave my notebook for future research as well.
  • Mark: as of 4/15/10 up to 30% recovery from 10 ng input sample with water elution. Also up to 13% recovery with 100ng/straw. 1-week trial completed as of 3/24. Results peak at 20% (-70C) and decrease with temperature. 2-month and 1-month trials complete, resulting in further degradation than in the 1-week trial. 4- and 6-month trials still in storage. Final Report draft submitted, due tomorrow. Presentation next Friday 4/30. Lab notebook will be updated and ready for future PATH SNAP workers.


‡ IIH Senior Project.

  • Ordered new HRP substrate. waiting for delivery
  • Will run ELISA in chip and image for standard curve.
  • finalizing report



Retrieved from "https://openwetware.org/mediawiki/index.php?title=Klapperich_Lab:Notebook/Lab_Meeting_Notes/2010/04/29&oldid=410900"