Klapperich Lab:Notebook/Lab Meeting Notes/2010/09/27

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> General Lab Meeting </span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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=27 September 2010 Lab Meeting=
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'''‡ Announcements'''<br>
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*New meeting time 2-4pm <br>
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'''†Trapping Mycobacteria (Jason)'''<br>
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* Bubble issue solved.<br>
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* Back to induction experiments. <br>
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<br>
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'''†Wave 80 project (Andy/Jane)'''<br>
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* Phenochloroform extraction of past exp flow throughs from SPE, including both particle and RNA input
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* Run bDNA on 1. HIV particles through straw 2. HIV particles boiled to lyze 3. flow through extracted samples
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<br>
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'''‡ Flu R01:Integration''' <br>
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* Paper for chip with Cathie. <br>
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* paper for biostatistics. Thinking phase Sonali.<br>
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* Sequence 10 more  products - for weekly QC.<br>
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* Run weekly negative gels. <br>
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<br>
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'''† HDA (Lead:Sonali)'''<br>
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* Start planning R01 for Submission on '''2/5/11.''' MM, CMK. <br>
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* <br>
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*
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<br>
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'''† Sample Concentration (Lead: Jane)''' <br>
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* phenol chloroform/etoh ppt method to "benchmark" Qiagen and SPE<br>
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<br>
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'''† SPE Column Optimization for DNA (Sam and Sonali)''' <br>
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* Mass spec results still sought, lower priority. <br>
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* sr project people will do silica optimization design/cassidy. <br>
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<br>
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'''‡C. diff Project''' (Cathie, Sonali, Satish Singh, Lisa J., His post doc )'''<br>
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* Shichu look for those old negatives in the box. <br>
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* Lisa is hounding Nancy Miller <br>
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* Will need more true negative stool samples. <br>
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<br>
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'''‡ Coulter Flu Fraunhofer Project (Sonali)
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* They still need RNA from us. <br>
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<br>
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'''‡ Automated Sample Preparation (Lead: Alex)'''<br>
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* Paper on HotDog - with CMK/ASB.  7/2 <br>
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<br>
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'''‡ Biointerfaces<br>
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'''* submitted. <br>'''
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<br>
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'''‡ CIMIT- Sepsis (Lead:Cathie, Team: Alex)'''<br>
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* New data with CMK. <br>
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<br>
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'''‡ PATH Grant (Lead:Cathie, Team:Jake, Samantha)<br>
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* New name for straws Me30Pd70 (M=monomer, e=EDMA, 30=30%, P=porogen, d=1-dodecanol, 70=70%) <br>
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* Finishing second round of 1-4 week storage studies on Me30Pd70; one week left <br>
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* New blood lysis procedures for blood with Tween 20 and Triton X-100 <br>
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* Moving all straw making to 709 from Fraunhofer <br>
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* Two full kits prepared, one left to go before extractions done on them <br>
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* Start senior project students on RNA optimization and silica optimization for DNA <br>
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* HIV particles run through three varieties of straws; bDNA assay and pcr analysis to come <br>
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* Chemical inventory done!!!  Enjoy <br>
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__NOTOC__
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