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| - | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> General Lab Meeting </span>
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| - | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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| - | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
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| - | =1 November 2010 Lab Meeting=
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| - | '''‡ Announcements'''<br>
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| - | * 2-4pm <br>
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| - | * NO More SCHEDULED PRESENTATIONS THIS SEMESTER. I will assign people week to week. Ignore names on Google calendar until I can fix it. <br>
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| - | * Jason K. will go next week (11/8). <br>
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| - | * This week (11/1), bring data that you want to discuss. We will try and keep this shortish (grant due F)<br>
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| - | '''†Trapping Mycobacteria (Jason)'''<br>
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| - | * <br>
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| - | '''†Nanomedicine'''<br>
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| - | * Dr. Rosen coming Tomorrow 11/2 to sort out assays. <br>
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| - | <br>
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| - | '''†Wave 80 project (Andy/Jane)'''<br>
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| - | * Making a scaled-up lot of ssDNA Pol standards, with gel purification / plasmid linearizations this time (AF)<br>
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| - | * Found better gag loci for real-time PCR; cloning plasmids for in vitro transcription RNA standards (AF)<br>
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| - | * Ran Boom buffer through monolith only straws. Will bDNA the eluent this week.(JZ)<br>
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| - | * Process flow throughs. (JZ) <br>
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| - | * Chentian - Figuring out a way to do silica channels. First pass: making plug with monolith. <br>
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| - | * ABI Taqman for HIV. Waiting for that to arrive. For LTR instead of gag. (MM) <br>
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| - | <br>
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| - | '''‡ Flu R01:Integration''' <br>
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| - | * Paper for chip with Cathie. <br>
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| - | * paper for biostatistics. Thinking phase Sonali.<br>
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| - | * Sequence 10 more products - for weekly QC.<br>
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| - | 10 products were sequenced, 2 of them failed. should we sequence 2 more?
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| - | * Run weekly negative gels. <br>
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| - | done
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| - | <br>
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| - | '''† HDA (Lead:Sonali)'''<br>
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| - | * Start planning R01 for Submission on '''2/5/11.''' MM, CMK. <br>
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| - | * <br>
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| - | *
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| - | <br>
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| - | '''† Sample Concentration (Lead: Jane)''' <br>
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| - | * phenol chloroform/etoh ppt method to "benchmark" Qiagen and SPE - done. PCR ongoing.<br>
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| - | <br>
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| - | '''† SPE Column Optimization for DNA (Sam and Sonali)''' <br>
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| - | * Mass spec results still sought, lower priority. <br>
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| - | * sr project people will do silica optimization design/cassidy. <br>
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| - | <br>
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| - | '''‡C. diff Project''' (Cathie, Sonali, Satish Singh, Lisa J., His post doc )'''<br>
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| - | * HDA in tube to test extracted "ELISA negative samples", the result is different from real-time PCR <br>
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| - | * Run gel to check real-time PCR products, product bands are really faint (different from before) <br>
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| - | * In the process of cloning HDA positive samples to check whether they are true positive <br>
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| - | * Run regular PCR with Sara’s old primers, results are inconsistent with HDA and real-time PCR <br>
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| - | <br>
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| - | '''‡ Coulter Flu Fraunhofer Project (Sonali)
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| - | * They still need RNA from us. <br>
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| - | <br>
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| - | '''‡ Automated Sample Preparation (Lead: Alex)'''<br>
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| - | * Paper on HotDog - with CMK/ASB. 7/2 <br>
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| - | <br>
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| - | '''‡ Biointerfaces<br>
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| - | '''* submitted. <br>'''
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| - | <br>
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| - | '''‡ CIMIT- Sepsis (Lead:Cathie, Team: Alex)'''<br>
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| - | * New data with CMK. <br>
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| - | <br>
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| - | '''‡ PATH Grant (Lead:Cathie, Team:Jake, Samantha)<br>
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| - | * New name for straws Me30Pd70 (M=monomer, e=EDMA, 30=30%, P=porogen, d=1-dodecanol, 70=70%) <br>
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| - | * Finishing second round of 1-4 week storage studies on Me30Pd70; one week left <br>
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| - | * New blood lysis procedures for blood with Tween 20 and Triton X-100 <br>
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| - | * Moving all straw making to 709 from Fraunhofer <br>
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| - | * Two full kits prepared, one left to go before extractions done on them <br>
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| - | * Start senior project students on RNA optimization and silica optimization for DNA <br>
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| - | * HIV particles run through three varieties of straws; bDNA assay and pcr analysis to come <br>
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| - | * Chemical inventory done!!! Enjoy <br>
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