15 November 2010 Lab Meeting

‡ Announcements

• 2-4pm
  
• Andy is up to talk.
  
• 
  

†Trapping Mycobacteria (Jason)

†Nanomedicine

†Wave 80 project (Andy/Jane)

• Making a scaled-up lot of ssDNA Pol standards, with gel purification / plasmid linearizations this time (AF)
  
• Found better gag loci for real-time PCR; cloning plasmids for in vitro transcription RNA standards (AF)
  
• Ran Boom buffer through monolith only straws. Will bDNA the eluent this week.(JZ)
  
• Process flow throughs. (JZ)
  
• Chentian - Figuring out a way to do silica channels. First pass: making plug with monolith.
  
• ABI Taqman for HIV. Waiting for that to arrive. For LTR instead of gag. (MM)
  

‡ Flu R01:Integration

• Paper for chip with Cathie.
  
• paper for biostatistics. Thinking phase Sonali.
  
• Sequence 10 more products - for weekly QC.
  

10 products were sequenced, 2 of them failed. should we sequence 2 more? 

• Run weekly negative gels.
  

 done

† HDA (Lead:Sonali)

• Start planning R01 for Submission on 2/5/11. MM, CMK.
  
• 
  

† Sample Concentration (Lead: Jane)

• Qiagen and SPE done in parallel on original and concentrated samples.
  
• New fixture sent to machine shop.
  
• New mask and mold in progress.

† SPE Column Optimization for DNA (Sam and Sonali)

• Mass spec results still sought, lower priority.
  
• sr project people will do silica optimization design/cassidy.
  

‡C. diff Project (Cathie, Sonali, Satish Singh, Lisa J., His post doc )

• HDA in tube to test extracted "ELISA negative samples", the result is different from real-time PCR
  
• Run gel to check real-time PCR products, product bands are really faint (different from before)
  
• In the process of cloning HDA positive samples to check whether they are true positive
  
• Run regular PCR with Sara’s old primers, results are inconsistent with HDA and real-time PCR
  

‡ Coulter Flu Fraunhofer Project (Sonali)

• They still need RNA from us.
  

‡ Automated Sample Preparation (Lead: Alex)

‡ Biointerfaces
* submitted.

‡ CIMIT- Sepsis (Lead:Cathie, Team: Alex)

• New data with CMK.
  

‡ PATH Grant (Lead:Cathie, Team:Jake, Samantha)

• New name for straws Me30Pd70 (M=monomer, e=EDMA, 30=30%, P=porogen, d=1-dodecanol, 70=70%)
  
• Finishing second round of 1-4 week storage studies on Me30Pd70; one week left
  
• New blood lysis procedures for blood with Tween 20 and Triton X-100
  
• Moving all straw making to 709 from Fraunhofer
  
• Two full kits prepared, one left to go before extractions done on them
  
• Start senior project students on RNA optimization and silica optimization for DNA
  
• HIV particles run through three varieties of straws; bDNA assay and pcr analysis to come
  
• Chemical inventory done!!! Enjoy