Klapperich Lab:Notebook/Lab Meeting Notes/2011/01/25

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Current revision (13:28, 25 January 2011) (view source)
 
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'''‡ Announcements'''<br>
'''‡ Announcements'''<br>
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* Cathie at BU nominally T and W. At MIT M, Th and F.
* 11:30-12:30 pm <br>
* 11:30-12:30 pm <br>
* REVIEW FOR THE NEW YEAR.
* REVIEW FOR THE NEW YEAR.
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'''† Sample Concentration (Lead: Jane, Nga)''' <br>
'''† Sample Concentration (Lead: Jane, Nga)''' <br>
* Meeting Fridays PHO 11-12pm. <br>
* Meeting Fridays PHO 11-12pm. <br>
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*
 
<br>
<br>
'''† SERS Franhofer Project (Jared, Chris, Jake, Cathie)''' <br>
'''† SERS Franhofer Project (Jared, Chris, Jake, Cathie)''' <br>
  * Meetings Wed 9-10am at Fraunhofer. <br>
  * Meetings Wed 9-10am at Fraunhofer. <br>
<br>
<br>
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'''‡C. diff Project''' (Cathie, Sonali, Satish Singh, Lisa J., His post doc )'''<br>
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'''‡C. diff Project''' (Cathie, Sonali, Satish, Shichu, QQ, Lisa J., Andy)'''<br>
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* Recall: Extracted "ELISA negative samples". They are HDA(toxin A) positive with HDA cloning oligos, but PCR(toxin A) negative with Sara's oligos, and Taqman real-time(toxin B) negative.  So right now is in the process of confirming above results and figuring out the reason for this <br>
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* Meeting times TBA.<br>
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* Repeat HDA with HDA cloning oligos on different day by using different aliquots of reagents.  The result is repeatable.  It can also be repeated by doing 2-step HDA reaction <br>
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* Clone and Seq. HDA positive samples, and they are true positive <br>
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* Optimize phusion enzyme PCR condition for patient samples.  Use optimized condition to PCR toxin A gene of more patient samples. Clone & Seq. these amplicons to check whether there is any deletion in toxin A gene of patient samples. <br>
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<br>
<br>
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'''‡ Coulter Flu Fraunhofer Project (Sonali)
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'''‡ CIMIT (actually two sepsis projects)- Sepsis (Cathie)'''<br>
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* They still need RNA from us. <br>
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<br>
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'''‡ Biointerfaces<br>
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'''* Accepted! <br>'''
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<br>
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'''‡ CIMIT- Sepsis (Lead:Cathie, Team: Alex)'''<br>
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* NEW person needed for this project. <br>
* NEW person needed for this project. <br>
* Negative control of 16s primers as well as of DXS primers (Sonali's Mix) show signals on qPCR. <br>
* Negative control of 16s primers as well as of DXS primers (Sonali's Mix) show signals on qPCR. <br>
<br>
<br>
'''‡ PATH Grant (Lead:Cathie, Team:Jake, Samantha)<br>
'''‡ PATH Grant (Lead:Cathie, Team:Jake, Samantha)<br>
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* Straw Extractions on prototypes beginning!!! Two down, four to go before analysis.
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* Meetings Wed 3-4 pm every other. <br>
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* Need to order more straws. Jake is working with Paul on that. <br>
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<br>
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* Working with potential filtering mechanism to prevent straw clogging from whole blood input. <br>
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* Begin storage studies using blood as input and spiked RNA.  Also will start spiking HIV versant standards into whole blood for storage. <br>
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* Extractions on straws using warmed (to 60C) NF water for elution. <br>
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Current revision

General Lab Meeting Main project page
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25 January 2011 Lab Meeting

‡ Announcements

  • Cathie at BU nominally T and W. At MIT M, Th and F.
  • 11:30-12:30 pm
  • REVIEW FOR THE NEW YEAR.


†Trapping Mycobacteria (Jason)

  • Spent last week in cleanroom, successfully made a new device.
  • Cells stay trapped better, as expected (don't have images yet)

†Nanomedicine (Sunil, Sonali, Cathie and Jen Rosen)

  • Sunil


†Wave 80 project (Andy/Sam/Sonali/Cathie)

  • Every other week meeting 2-3:30 SKPYE.


‡ Flu R01:Integration

  • Paper drafting v.8
  • Grant renewal will be reviewed on 1/27.


† HDA (Lead:Sonali)

  • submitted erratum 1/24/11.


† Sample Concentration (Lead: Jane, Nga)

  • Meeting Fridays PHO 11-12pm.


† SERS Franhofer Project (Jared, Chris, Jake, Cathie) 

* Meetings Wed 9-10am at Fraunhofer.


‡C. diff Project (Cathie, Sonali, Satish, Shichu, QQ, Lisa J., Andy)

  • Meeting times TBA.


‡ CIMIT (actually two sepsis projects)- Sepsis (Cathie)

  • NEW person needed for this project.
  • Negative control of 16s primers as well as of DXS primers (Sonali's Mix) show signals on qPCR.


‡ PATH Grant (Lead:Cathie, Team:Jake, Samantha)

  • Meetings Wed 3-4 pm every other.



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