25 January 2011 Lab Meeting
‡ Announcements
- 11:30-12:30 pm
- REVIEW FOR THE NEW YEAR.
†Trapping Mycobacteria (Jason)
- Spent last week in cleanroom, successfully made a new device.
- Cells stay trapped better, as expected (don't have images yet)
†Nanomedicine (Sunil, Sonali, Cathie and Jen Rosen)
†Wave 80 project (Andy/Sam/Sonali/Cathie)
- Every other week meeting 2-3:30 SKPYE.
‡ Flu R01:Integration
- Paper drafting v.8
- Grant renewal will be reviewed on 1/27.
† HDA (Lead:Sonali)
- submitted erratum 1/24/11.
† Sample Concentration (Lead: Jane, Nga)
- Meeting Fridays PHO 11-12pm.
† SERS Franhofer Project (Jared, Chris, Jake, Cathie)
* Meetings Wed 9-10am at Fraunhofer.
‡C. diff Project (Cathie, Sonali, Satish Singh, Lisa J., His post doc )
- Recall: Extracted "ELISA negative samples". They are HDA(toxin A) positive with HDA cloning oligos, but PCR(toxin A) negative with Sara's oligos, and Taqman real-time(toxin B) negative. So right now is in the process of confirming above results and figuring out the reason for this
- Repeat HDA with HDA cloning oligos on different day by using different aliquots of reagents. The result is repeatable. It can also be repeated by doing 2-step HDA reaction
- Clone and Seq. HDA positive samples, and they are true positive
- Optimize phusion enzyme PCR condition for patient samples. Use optimized condition to PCR toxin A gene of more patient samples. Clone & Seq. these amplicons to check whether there is any deletion in toxin A gene of patient samples.
‡ Coulter Flu Fraunhofer Project (Sonali)
- They still need RNA from us.
‡ Biointerfaces
* Accepted!
‡ CIMIT- Sepsis (Lead:Cathie, Team: Alex)
- NEW person needed for this project.
- Negative control of 16s primers as well as of DXS primers (Sonali's Mix) show signals on qPCR.
‡ PATH Grant (Lead:Cathie, Team:Jake, Samantha)
- Straw Extractions on prototypes beginning!!! Two down, four to go before analysis.
- Need to order more straws. Jake is working with Paul on that.
- Working with potential filtering mechanism to prevent straw clogging from whole blood input.
- Begin storage studies using blood as input and spiked RNA. Also will start spiking HIV versant standards into whole blood for storage.
- Extractions on straws using warmed (to 60C) NF water for elution.
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General Lab Meeting
