Klapperich Lab:Photografting to Zeonor Slides

Overview

This protocol describes the procedure for grafting PEG-acrylate to Zeonor slides.

Materials

• PEG acrylate
• Ethylene Glycol
• 2-Dimethoxy-2-acetophenone (DMPAP)
• Zeonor slides
• methanol

Procedure

1. Weigh out 35 mg DMPAP
2. Clean plastic slide with EtOH, then rinse with Millipore water
3. Plasma treat surface of plastic slide for 2 min on medium
4. Mix DMPAP with 800[math]\displaystyle{ u }[/math]L Ethylene Glycol and 1200[math]\displaystyle{ u }[/math]L PEG-acrylate in 2mL tube to get 60% PEG-acrylate solution
5. Heat solution in hot water bath at 85&degC until DMPAP crystals have melted
6. Vortex solution
7. Add solution in uniform layer plastic slide
8. Photograft slide in UV Crosslinker for 30 seconds on maximum energy
9. Rinse with MeOH and let dry under hood
10. remove excess poly-PEG acrylate

Notes

1. List troubleshooting tips here.
2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
3. Anecdotal observations that might be of use to others can also be posted here.

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References

Relevant papers and books

1. Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 |
2. JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 |
3. ISBN:0879697164

Contact

• Aisha