Klapperich Lab:Photopolymerization of PHEMA: Difference between revisions

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==Materials==
==Materials==
List reagents, supplies and equipment necessary to perform the protocol here.  For those materials which have their own OWW pages, link to that page.  Alternatively, links to the suppliers' page on that material are also appropriate.
*900 uL HEMA or modified monomer
 
*600 uL Ethylene glycol
*supply 1 (i.e. tubes of a certain size? spreaders?)
*25 uL EGDMA (ethylene glycol dimethacrylate), crosslinker
*reagent 1
*20 mg DMPAP, photoinitiator
*X μL reagent 2
**component A (reagent 2 is made up of multiple components)
**component B
*equipment 1
*equipment 2


==Procedure==
==Procedure==
#Step 1
#Turn on digital dry bath.  Add water to wells you will use. Set temperature to 85°C.
#Step 2
#Add DMPAP to 2 mL centrifuge tube.
#*Step 2 has some additional information that goes with it.  i.e. Keep at 4°C.
#Add HEMA to tube.  Be careful to avoid air bubbles when pipetting.
#Step 3
#Add ethylene glycol to tube.
##Step 3 has multiple sub-steps within it.
#Add EGMDA to tube.
##Enumerate each of those.
#Heat mixture in digital bath until crystals are melted. Vortex.
#Add contents of tube to one well of a 6 well plate.  Expose to UV light for 2.5 minutes.


==Notes==
==Notes==
#List troubleshooting tips here.
#Be careful not to overheat DMPAP. <font face="courier"><nowiki>(Jessica Kaufman)</nowiki></font>
#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
#Let gel sit in fume hood for at least 4 hours before hydrating with Millipore water.<font face="courier"><nowiki>(Jessica Kaufman)</nowiki></font>
#Anecdotal observations that might be of use to others can also be posted here.
#This recipe in this mold yields a swollen gel that is approximately 1 mm thick.<font face="courier"><nowiki>(Jessica Kaufman)</nowiki></font>
 
Please sign your name to your note by adding <font face="courier"><nowiki>('''~~~~''')</nowiki></font> to the end of your tip.


==References==
==References==
'''Relevant papers and books'''
<!-- If this protocol has papers or books associated with it, list those references here.  See the [[OpenWetWare:Biblio]] page for more information. -->
<biblio>
<biblio>
#Goldbeter-PNAS-1981 pmid=6947258
#Song2005 pmid=15755154
#Jacob-JMB-1961 pmid=13718526
#Song2003 pmid=12553825
#Ptashne-Genetic-Switch isbn=0879697164
</biblio>
</biblio>


==Contact==
==Contact==
*Who has experience with this protocol?
*Jessica Kaufman
*Nathan Spencer
*Eben Tessari

Latest revision as of 11:03, 26 June 2006

Overview

This protocol outlines the photopolymerization of PHEMA.

Materials

  • 900 uL HEMA or modified monomer
  • 600 uL Ethylene glycol
  • 25 uL EGDMA (ethylene glycol dimethacrylate), crosslinker
  • 20 mg DMPAP, photoinitiator

Procedure

  1. Turn on digital dry bath. Add water to wells you will use. Set temperature to 85°C.
  2. Add DMPAP to 2 mL centrifuge tube.
  3. Add HEMA to tube. Be careful to avoid air bubbles when pipetting.
  4. Add ethylene glycol to tube.
  5. Add EGMDA to tube.
  6. Heat mixture in digital bath until crystals are melted. Vortex.
  7. Add contents of tube to one well of a 6 well plate. Expose to UV light for 2.5 minutes.

Notes

  1. Be careful not to overheat DMPAP. (Jessica Kaufman)
  2. Let gel sit in fume hood for at least 4 hours before hydrating with Millipore water.(Jessica Kaufman)
  3. This recipe in this mold yields a swollen gel that is approximately 1 mm thick.(Jessica Kaufman)

References

  1. Song J, Malathong V, and Bertozzi CR. Mineralization of synthetic polymer scaffolds: a bottom-up approach for the development of artificial bone. J Am Chem Soc. 2005 Mar 16;127(10):3366-72. DOI:10.1021/ja043776z | PubMed ID:15755154 | HubMed [Song2005]
  2. Song J, Saiz E, and Bertozzi CR. A new approach to mineralization of biocompatible hydrogel scaffolds: an efficient process toward 3-dimensional bonelike composites. J Am Chem Soc. 2003 Feb 5;125(5):1236-43. DOI:10.1021/ja028559h | PubMed ID:12553825 | HubMed [Song2003]

All Medline abstracts: PubMed | HubMed

Contact

  • Jessica Kaufman
  • Nathan Spencer
  • Eben Tessari
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