Klapperich Lab:Primary Neonatal Foreskin Fibroblasts

Overview

Replace this sentence with a brief description of the protocol and its goal.

Materials

Human neonatal foreskin from circumcisions performed at Brigham and Women's hospital are obtained. The foreskins are washed exhaustively in PBS and then may be stored in media at 4 C for up to 24 hours after excision. The epidermal and dermal layers are pulled apart using forceps and scalpel and the epidermis is reserved for other applications. The dermal layer is then cut in 2 mm2 sections and placed in media for transport to our lab.

Procedure

1. Step 1
2. Step 2
   • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
3. Step 3
   1. Step 3 has multiple sub-steps within it.
   2. Enumerate each of those.

Notes

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References

Relevant papers and books

1. Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 |
2. JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 |
3. ISBN:0879697164

Contact

• Who has experience with this protocol?