Knight:Evolving Reshmaverters

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Revision as of 03:26, 24 March 2006 by Jason R. Kelly (Talk | contribs)
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Goal

Try to use the screening plasmid to tune the promoters (or RBSs) of Reshmaverters to get an appropriate transfer curve.

Stages

  1. Design/Order/DS promoter library & Construct screening plamid + RBS/Rep/Term
  2. Insert promoter librarty into screening plasmid w/ inverter and screen on FACS
  3. Design/Order/DS RBS library & Construct screening plasmid + Rep/Term/Promoter
  4. Insert RBS librarty into screening plasmid w/ inverter and screen on FACS
Note: If 1/2 work then great, but probably a good idea to start 3 in parallel with 2 just in case.
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