Try to use the screening plasmid to tune the promoters (or RBSs) of Reshmaverters to get an appropriate transfer curve.
- Design/Order/DS promoter library & Construct screening plamid + RBS/Rep/Term
- Insert promoter librarty into screening plasmid w/ inverter and screen on FACS
- Design/Order/DS RBS library & Construct screening plasmid + Rep/Term/Promoter
- Insert RBS librarty into screening plasmid w/ inverter and screen on FACS
- Note: If 1/2 work then great, but probably a good idea to start 3 in parallel with 2 just in case.