Knight:Freezer: Difference between revisions
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Box Numbering: numeric shelf (top to bottom), alpha rack (left to right), numeric drawer (top to bottom), alpha box (front to back) | <font color="red">'''When you create a new box, label it on all four sides so that the label can be seen regardless of which orientation the box is put back in the freezer.'''</font> | ||
==Large -20°C freezer== | |||
'''Box Numbering: numeric shelf (top to bottom), alpha rack (left to right), numeric drawer (top to bottom), alpha box (front to back)''' | |||
{| border=1 | {| border=1 | ||
! | ! | ||
!Rack a | !Rack a | ||
Line 10: | Line 13: | ||
|---- bgcolor=silver | |---- bgcolor=silver | ||
!style="background:white" |Shelf 1 | !style="background:white" |Shelf 1 | ||
| | | <font color="red">'''iGEM, UROPs and Julie'''</font> | ||
{| border=1 | {| border=1 | ||
|---- bgcolor=gray | |---- bgcolor=gray | ||
|1a1a | |1a1a <br>Restriction enzymes | ||
|1a1b | |1a1b <br> MIT iGEM | ||
|1a1c | |1a1c <br>iGEM primers | ||
|---- | |---- | ||
|1a2a | |1a2a | ||
|1a2b | |1a2b | ||
|1a2c | |1a2c <br>iGEM/Randy primers? | ||
|---- bgcolor=gray | |---- bgcolor=gray | ||
|1a3a | |1a3a <br>Julie | ||
|1a3b | |1a3b <br>Julie | ||
|1a3c | |1a3c <br>Julie's -20°C box | ||
|---- | |---- | ||
|1a4a | |1a4a <br>Julie's -20°C box 2 | ||
|1a4b | |1a4b <br>Julie KAN | ||
|1a4c | |1a4c <br>Julie's oligos and other stuff | ||
|} | |} | ||
| | | | ||
| | | | ||
| <font color="red">'''Meagan and Austin'''</font> | |||
{| border=1 | {| border=1 | ||
|---- bgcolor=gray | |---- bgcolor=gray | ||
|1d1a | |1d1a | ||
|1d1b | |1d1b Meagan's -20 box | ||
|1d1c | |1d1c | ||
|---- | |---- | ||
|1d2a | |1d2a <br>Lysozyme | ||
|1d2b | |1d2b <br>Buffers | ||
|1d2c | |1d2c | ||
|---- bgcolor=gray | |---- bgcolor=gray | ||
|1d3a <br> | |1d3a <br>Pyrrolo-dC oligos | ||
|1d3b <br> | |1d3b <br>Transcription | ||
|1d3c <br> | |1d3c <br>Pyrophosphate assay | ||
|---- | |---- | ||
|1d4a | |1d4a <br>Austin's Box | ||
|1d4b | |1d4b <br>reserved for Austin | ||
|1d4c | |1d4c <br>Non-useful primers | ||
|} | |} | ||
|---- bgcolor=silver | |---- bgcolor=silver | ||
Line 103: | Line 70: | ||
|2a3c <br>plasmid engineering oligos | |2a3c <br>plasmid engineering oligos | ||
|---- | |---- | ||
|2a4a <br> | |2a4a <br>STF subassemblies | ||
|2a4b <br> | |2a4b <br>Device inputs | ||
|2a4c <br> | |2a4c <br>Device low state | ||
|} | |} | ||
| <font color="red">'''Tom'''</font> | | <font color="red">'''Tom'''</font> | ||
Line 111: | Line 78: | ||
|---- bgcolor=gray | |---- bgcolor=gray | ||
|2b1a <br>Three antibiotic plasmid PCR | |2b1a <br>Three antibiotic plasmid PCR | ||
|2b1b | |2b1b <br>Mesoplasma florum Transposases | ||
|2b1c | |2b1c | ||
|---- | |---- | ||
|2b2a | |2b2a <br> Misc box | ||
|2b2b | |2b2b | ||
|2b2c | |2b2c | ||
Line 133: | Line 100: | ||
|2c1c | |2c1c | ||
|---- | |---- | ||
|2c2a <br> | |2c2a <br>Restriction Enzymes 1 (A-B) | ||
|2c2b | |2c2b <br>Restriction Enzymes 2 (C-N) | ||
|2c2c | |2c2c <br>Restriction Enzymes 3 (O-Z) | ||
|---- bgcolor=gray | |---- bgcolor=gray | ||
|2c3a <br>Polymerases | |2c3a <br>Polymerases | ||
|2c3b | |2c3b <br>BioBricks enzymes and buffers | ||
|2c3c | |2c3c <br>DNA and Protein ladders | ||
|---- | |---- | ||
|2c4a | |2c4a <br>Ligases, phosphatases and kinases | ||
|2c4b | |2c4b <br>Antibiotic medium prep stocks | ||
|2c4c | |2c4c <br>RNAses and DNAses | ||
|} | |} | ||
| <font color="red">'''Austin'''</font> | | <font color="red">'''Austin'''</font> | ||
{| border=1 | {| border=1 | ||
|---- bgcolor=gray | |---- bgcolor=gray | ||
|2d1a <br> | |2d1a <br>Ribozyme Constructs | ||
|2d1b <br> | |2d1b <br>Ribozyme | ||
|2d1c <br> | |2d1c <br>Ribozyme Primers | ||
|---- | |---- | ||
|2d2a <br> | |2d2a <br>BioBricks++ Plasmids | ||
|2d2b <br> | |2d2b <br>BioBricks++ Parts | ||
|2d2c <br> | |2d2c <br>BioBricks++ Primers | ||
|---- bgcolor=gray | |---- bgcolor=gray | ||
|2d3a <br> | |2d3a <br>BioBricks Plasmids | ||
|2d3b <br> | |2d3b <br>BioBricks Parts | ||
|2d3c <br>Austin | |2d3c <br>Austin's Primers | ||
|---- | |---- | ||
|2d4a <br> | |2d4a <br>Light-control | ||
|2d4b <br> | |2d4b <br>Promoters | ||
|2d4c <br> | |2d4c <br>Empty | ||
|} | |} | ||
|---- bgcolor=silver | |---- bgcolor=silver | ||
Line 171: | Line 138: | ||
|3a1a | |3a1a | ||
|3a1b | |3a1b | ||
|3a1c Dig/Biotin labelled dNTPS, markers | |3a1c <br>Dig/Biotin labelled dNTPS, markers | ||
|---- | |---- | ||
|3a2a <br>Randy's box | |3a2a <br>Randy's box | ||
Line 181: | Line 148: | ||
|3a3c <br>arcT and unc 3030 dig probes | |3a3c <br>arcT and unc 3030 dig probes | ||
|---- | |---- | ||
|3a4a <br> | |3a4a <br> | ||
|3a4b <br> | |3a4b <br> | ||
|3a4c <br> | |3a4c <br> | ||
|} | |} | ||
| | | | ||
| | | | ||
| <font color="red">'''Misc'''</font> | |||
{| border=1 | {| border=1 | ||
|---- bgcolor=gray | |---- bgcolor=gray | ||
|3d1a | |3d1a <br>NEBuffers 1,2,3,4, BSA, MgSO<sub>4</sub> | ||
|3d1b | |3d1b <br> | ||
|3d1c | |3d1c <br> | ||
|---- | |---- | ||
|3d2a | |3d2a <br> | ||
|3d2b | |3d2b <br> | ||
|3d2c | |3d2c <br> | ||
|---- bgcolor=gray | |---- bgcolor=gray | ||
|3d3a | |3d3a <br> | ||
|3d3b | |3d3b <br> | ||
|3d3c | |3d3c <br> | ||
|---- | |---- | ||
|3d4a | |3d4a | ||
Line 247: | Line 178: | ||
| <font size="6">Coolers</font> | | <font size="6">Coolers</font> | ||
| | | | ||
| | | | ||
|} | |} | ||
==Reagents stored in boxes== | |||
* Amphoteracin B solution (Sigma) | |||
* Antibiotic Antimycotic solution (Sigma) | |||
* ATP (Amersham) 100 mM solution | |||
* ATP A7699 1g | |||
* L-Arginininamide A3913 | |||
* BCIP fast tablets B5655 | |||
* BCIP tablets B6024 | |||
* Benchmark HIS tagged protein standards | |||
* BSA A7030 | |||
* bis-(2-mercaptoethyl) sulfone [NucleixPlus] | |||
* Catalase C9322 | |||
* Catalase C40 | |||
* dl-p-chlorophenylalanine Aldrich 130710 | |||
* dl-p-chlorophenylalanine C6506 | |||
* N6 2' o-dibutyrl adenosine 3':5' cyclic monophosphate D0627 | |||
* DIG Chem-link (Roche) | |||
* DIG-11-dUTP (Roche) | |||
* DNAse I RNAse free Roche | |||
* DTT (NEB) | |||
* DTT | |||
* 5' fluorescein phosphoramidite (2x) | |||
* Iodoacetic acid I9148 | |||
* IPTG (5g) | |||
* a-Ketoglutaric acid K1128 | |||
* Lysozyme L6876 | |||
* 4-methylumbeliferyl B-D-glucopyranoside | |||
* 4-methylumbeliferyl b-D-glucosaminide | |||
* N-(beta-keto caproyl) L-homoserine lactone K3007 | |||
* N-acetyl glucosamine A3286 | |||
* b-NAD N7004 | |||
* Oleic acid O1630 | |||
* Oligo dT-cellulose O3131 | |||
* PABA A9878 | |||
* d-Panothenic Acid P2250 | |||
* Pellet Paint NF (Novagen) | |||
* Phenol, acid Ambion | |||
* Protease P6911 | |||
* Protease inhibitor cocktail P8465 | |||
* Proteinase K (shelton scientific) | |||
* Pyridoxal P9130 | |||
* Pyridoxaminhe P9380 | |||
* Ribonuclease A R4875 | |||
* Ribonuclease T1 R1003 | |||
* Rifampicin | |||
* RNA Vanadyl complexes R3380 | |||
* RNAse, DNAse free Boehringer-Mannheim 1119915 | |||
* RNAse A R4642 | |||
* Spermidine S2501 | |||
* Spermine S1141 (2x) | |||
* Stratagene Perfect Match PCR enhancer | |||
* Streptavidin S4762 | |||
* SYBR Gold stain | |||
* SybrGreen I nucleic acid stain | |||
* SybrGreen II RNA stain | |||
* Tetracycline | |||
* Transfer RNA R8759 |
Latest revision as of 09:01, 11 January 2008
When you create a new box, label it on all four sides so that the label can be seen regardless of which orientation the box is put back in the freezer.
Large -20°C freezer
Box Numbering: numeric shelf (top to bottom), alpha rack (left to right), numeric drawer (top to bottom), alpha box (front to back)
Rack a | Rack b | Rack c | Rack d | |||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Shelf 1 | iGEM, UROPs and Julie
|
Meagan and Austin
| ||||||||||||||||||||||||||||||||||||||||||||||||||
Shelf 2 | Reshma
|
Tom
|
Common lab reagents
|
Austin
| ||||||||||||||||||||||||||||||||||||||||||||||||
Shelf 3 | Old stuff
|
Misc
| ||||||||||||||||||||||||||||||||||||||||||||||||||
Shelf 4 | Coolers | Coolers |
Reagents stored in boxes
- Amphoteracin B solution (Sigma)
- Antibiotic Antimycotic solution (Sigma)
- ATP (Amersham) 100 mM solution
- ATP A7699 1g
- L-Arginininamide A3913
- BCIP fast tablets B5655
- BCIP tablets B6024
- Benchmark HIS tagged protein standards
- BSA A7030
- bis-(2-mercaptoethyl) sulfone [NucleixPlus]
- Catalase C9322
- Catalase C40
- dl-p-chlorophenylalanine Aldrich 130710
- dl-p-chlorophenylalanine C6506
- N6 2' o-dibutyrl adenosine 3':5' cyclic monophosphate D0627
- DIG Chem-link (Roche)
- DIG-11-dUTP (Roche)
- DNAse I RNAse free Roche
- DTT (NEB)
- DTT
- 5' fluorescein phosphoramidite (2x)
- Iodoacetic acid I9148
- IPTG (5g)
- a-Ketoglutaric acid K1128
- Lysozyme L6876
- 4-methylumbeliferyl B-D-glucopyranoside
- 4-methylumbeliferyl b-D-glucosaminide
- N-(beta-keto caproyl) L-homoserine lactone K3007
- N-acetyl glucosamine A3286
- b-NAD N7004
- Oleic acid O1630
- Oligo dT-cellulose O3131
- PABA A9878
- d-Panothenic Acid P2250
- Pellet Paint NF (Novagen)
- Phenol, acid Ambion
- Protease P6911
- Protease inhibitor cocktail P8465
- Proteinase K (shelton scientific)
- Pyridoxal P9130
- Pyridoxaminhe P9380
- Ribonuclease A R4875
- Ribonuclease T1 R1003
- Rifampicin
- RNA Vanadyl complexes R3380
- RNAse, DNAse free Boehringer-Mannheim 1119915
- RNAse A R4642
- Spermidine S2501
- Spermine S1141 (2x)
- Stratagene Perfect Match PCR enhancer
- Streptavidin S4762
- SYBR Gold stain
- SybrGreen I nucleic acid stain
- SybrGreen II RNA stain
- Tetracycline
- Transfer RNA R8759