Knight:Freezer

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When you create a new box, label it on all four sides so that the label can be seen regardless of which orientation the box is put back in the freezer.

Large -20°C freezer

Box Numbering: numeric shelf (top to bottom), alpha rack (left to right), numeric drawer (top to bottom), alpha box (front to back)

Rack a Rack b Rack c Rack d
Shelf 1 iGEM, UROPs and Julie
1a1a
Restriction enzymes
1a1b
MIT iGEM
1a1c
iGEM primers
1a2a 1a2b 1a2c
iGEM/Randy primers?
1a3a
Julie
1a3b
Julie
1a3c
Julie's -20°C box
1a4a
Julie's -20°C box 2
1a4b
Julie KAN
1a4c
Julie's oligos and other stuff
Meagan and Austin
1d1a 1d1b Meagan's -20 box 1d1c
1d2a
Lysozyme
1d2b
Buffers
1d2c
1d3a
Pyrrolo-dC oligos
1d3b
Transcription
1d3c
Pyrophosphate assay
1d4a
Austin's Box
1d4b
reserved for Austin
1d4c
Non-useful primers
Shelf 2 Reshma
2a1a
synthetic transcription factors
2a1b
STF: device outputs
2a1c
STF: oligos
2a2a
working
2a2b
crosstalk assemblies
2a2c
crosstalk parts and subassemblies
2a3a
STF: device outputs with readout
2a3b
plasmid engineering
2a3c
plasmid engineering oligos
2a4a
STF subassemblies
2a4b
Device inputs
2a4c
Device low state
Tom
2b1a
Three antibiotic plasmid PCR
2b1b
Mesoplasma florum Transposases
2b1c
2b2a
Misc box
2b2b 2b2c
2b3a 2b3b 2b3c
2b4a 2b4b 2b4c
Common lab reagents
2c1a
Common primers
2c1b
Common DNA
2c1c
2c2a
Restriction Enzymes 1 (A-B)
2c2b
Restriction Enzymes 2 (C-N)
2c2c
Restriction Enzymes 3 (O-Z)
2c3a
Polymerases
2c3b
BioBricks enzymes and buffers
2c3c
DNA and Protein ladders
2c4a
Ligases, phosphatases and kinases
2c4b
Antibiotic medium prep stocks
2c4c
RNAses and DNAses
Austin
2d1a
Ribozyme Constructs
2d1b
Ribozyme
2d1c
Ribozyme Primers
2d2a
BioBricks++ Plasmids
2d2b
BioBricks++ Parts
2d2c
BioBricks++ Primers
2d3a
BioBricks Plasmids
2d3b
BioBricks Parts
2d3c
Austin's Primers
2d4a
Light-control
2d4b
Promoters
2d4c
Empty
Shelf 3 Old stuff
3a1a 3a1b 3a1c
Dig/Biotin labelled dNTPS, markers
3a2a
Randy's box
3a2b
Randy BioBricks
3a2c
3a3a
lacZ gene synthesis
3a3b
cI and derivatives
3a3c
arcT and unc 3030 dig probes
3a4a
3a4b
3a4c
Misc
3d1a
NEBuffers 1,2,3,4, BSA, MgSO4
3d1b
3d1c
3d2a
3d2b
3d2c
3d3a
3d3b
3d3c
3d4a 3d4b 3d4c
Shelf 4 Coolers Coolers


Reagents stored in boxes

  • Amphoteracin B solution (Sigma)
  • Antibiotic Antimycotic solution (Sigma)
  • ATP (Amersham) 100 mM solution
  • ATP A7699 1g
  • L-Arginininamide A3913
  • BCIP fast tablets B5655
  • BCIP tablets B6024
  • Benchmark HIS tagged protein standards
  • BSA A7030
  • bis-(2-mercaptoethyl) sulfone [NucleixPlus]
  • Catalase C9322
  • Catalase C40
  • dl-p-chlorophenylalanine Aldrich 130710
  • dl-p-chlorophenylalanine C6506
  • N6 2' o-dibutyrl adenosine 3':5' cyclic monophosphate D0627
  • DIG Chem-link (Roche)
  • DIG-11-dUTP (Roche)
  • DNAse I RNAse free Roche
  • DTT (NEB)
  • DTT
  • 5' fluorescein phosphoramidite (2x)
  • Iodoacetic acid I9148
  • IPTG (5g)
  • a-Ketoglutaric acid K1128
  • Lysozyme L6876
  • 4-methylumbeliferyl B-D-glucopyranoside
  • 4-methylumbeliferyl b-D-glucosaminide
  • N-(beta-keto caproyl) L-homoserine lactone K3007
  • N-acetyl glucosamine A3286
  • b-NAD N7004
  • Oleic acid O1630
  • Oligo dT-cellulose O3131
  • PABA A9878
  • d-Panothenic Acid P2250
  • Pellet Paint NF (Novagen)
  • Phenol, acid Ambion
  • Protease P6911
  • Protease inhibitor cocktail P8465
  • Proteinase K (shelton scientific)
  • Pyridoxal P9130
  • Pyridoxaminhe P9380
  • Ribonuclease A R4875
  • Ribonuclease T1 R1003
  • Rifampicin
  • RNA Vanadyl complexes R3380
  • RNAse, DNAse free Boehringer-Mannheim 1119915
  • RNAse A R4642
  • Spermidine S2501
  • Spermine S1141 (2x)
  • Stratagene Perfect Match PCR enhancer
  • Streptavidin S4762
  • SYBR Gold stain
  • SybrGreen I nucleic acid stain
  • SybrGreen II RNA stain
  • Tetracycline
  • Transfer RNA R8759
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