Knight:Materials

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(Common primers)
Current revision (11:37, 3 October 2009) (view source)
m (Reverted edits by Li Miao (Talk) to last version by Reshma P. Shetty)
 
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==Common primers==
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==Dry ice==
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===VF2===
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Dry ice is delivered on Tuesday mornings (before noon).  The stash usually lasts all week but is gone some time over the weekend.  There's not any remaining by Monday morning.
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5' tgc cac ctg acg tct aag aa 3'
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==DNA==
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===Primers===
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See [[Knight:Primers]].
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===VR===
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===Electrophoresis===
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*[[Knight:Loading dye]]
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5' ATT ACC GCC TTT GAG TGA GC 3'
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==RNA==
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*[[Knight:RNA work]]
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===BioBricks-f===
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==Protein==
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*[http://www.piercenet.com/products/browse.cfm?fldID=02020105 Coomassie (Bradford) Protein Assay Kit] from Pierce
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*[http://www.piercenet.com/products/browse.cfm?fldID=02020108 Albumin standard] from Pierce
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*[http://www.invitrogen.com/content/sfs/manuals/invisionstain_man.pdf InVision™ His-tag In-gel Stain] from Invitrogen (purchased in 2003?)
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  5' GTT TCT TCG AAT TCG CGG CCG CTT CTA G 3'
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===Quartz cuvettes for CD===
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For our old spectrophotometer, we had two macrocell QS quartz cuvettes and two microcell QS quartz cuvettes. I tested the quality of these four cuvettes for the purposes of CD (circular dichroism) using the [http://csbi.mit.edu/biophysinstrument/cdspectrometer CD spectrometer] at the [http://csbi.mit.edu/biophysinstrument/ Bioinstrumentation facility].  The two microcell cuvettes were not suitable for CD ... they have high birefringence.  The two macrocell cuvettes were suitable for CD in the wavelenth range 224-320nm.  However, for protein structures like alpha helices, you'll likely want to check the spectra even at 200nm.  So these cuvettes are not great for this purpose.  A cuvette for use in CD was purchased.
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*ordered by [[Reshma Shetty | Reshma]] 5/1/2006 (25&mu;M)
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==Stocks==
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*[[Knight:Dyes]]
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===BioBricks-r===
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*[[Knight:Freezer]]
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*[[Knight:Fridge]]
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5' GTT TCT TCC TGC AGC GGC CGC TAC TAG TA 3'
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*[[Knight:yeast plasmids|Plasmids]]
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*[[Knight:yeast strains|Strains]]
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*ordered by [[Reshma Shetty | Reshma]] 8/24/2006
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===M13-R===
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5' cag gaa aca gct atg ac 3'
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*ordered by [[Austin Che | Austin]] (50&mu;M)
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===M13-F (-20)===
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5' GTA AAA CGA CGG CCA G 3'
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*ordered by [[Austin Che | Austin]] (50&mu;M)
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==Dry ice==
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Dry ice is delivered on Tuesday mornings (before noon).  The stash usually lasts all week but is gone some time over the weekend.  There's not any remaining by Monday morning.
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==Other==
 
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[[Knight:Freezer]]
 
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Current revision

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Knight Lab



Contents

Dry ice

Dry ice is delivered on Tuesday mornings (before noon). The stash usually lasts all week but is gone some time over the weekend. There's not any remaining by Monday morning.

DNA

Primers

See Knight:Primers.

Electrophoresis

RNA

Protein

Quartz cuvettes for CD

For our old spectrophotometer, we had two macrocell QS quartz cuvettes and two microcell QS quartz cuvettes. I tested the quality of these four cuvettes for the purposes of CD (circular dichroism) using the CD spectrometer at the Bioinstrumentation facility. The two microcell cuvettes were not suitable for CD ... they have high birefringence. The two macrocell cuvettes were suitable for CD in the wavelenth range 224-320nm. However, for protein structures like alpha helices, you'll likely want to check the spectra even at 200nm. So these cuvettes are not great for this purpose. A cuvette for use in CD was purchased.

Stocks


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