Knight:Materials

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==Quartz cuvettes==
==Quartz cuvettes==
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For our old spectrophotometer, we had two macrocell QS quartz cuvettes and two microcell QS quartz cuvettes.  I tested the quality of these four cuvettes for the purposes of CD (circular dichroism) using the [http://csbi.mit.edu/biophysinstrument/cdspectrometer CD spectrometer] at the [http://csbi.mit.edu/biophysinstrument/ Bioinstrumentation facility].  The two microcell cuvettes were not suitable for CD ... they have high birefringence.  The two macrocell cuvettes were suitable for CD in the wavelenth range 224-320nm.  However, for protein structures like alpha helices, you'll likely want to check the spectra even at 200nm.  So these cuvettes are not great for this purpose.
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For our old spectrophotometer, we had two macrocell QS quartz cuvettes and two microcell QS quartz cuvettes.  I tested the quality of these four cuvettes for the purposes of CD (circular dichroism) using the [http://csbi.mit.edu/biophysinstrument/cdspectrometer CD spectrometer] at the [http://csbi.mit.edu/biophysinstrument/ Bioinstrumentation facility].  The two microcell cuvettes were not suitable for CD ... they have high birefringence.  The two macrocell cuvettes were suitable for CD in the wavelenth range 224-320nm.  However, for protein structures like alpha helices, you'll likely want to check the spectra even at 200nm.  So these cuvettes are not great for this purpose.  A cuvette for use in CD was purchased.
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Revision as of 17:39, 27 November 2006

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Knight Lab



Contents

Common primers

VF2

5' tgc cac ctg acg tct aag aa 3'

VR

5' ATT ACC GCC TTT GAG TGA GC 3'

BioBricks-f

5' GTT TCT TCG AAT TCG CGG CCG CTT CTA G 3'
  • ordered by Reshma 5/1/2006 (25μM)

BioBricks-r

5' GTT TCT TCC TGC AGC GGC CGC TAC TAG TA 3'

M13-R

5' cag gaa aca gct atg ac 3'

M13-F (-20)

5' GTA AAA CGA CGG CCA G 3'

Protein

Dry ice

Dry ice is delivered on Tuesday mornings (before noon). The stash usually lasts all week but is gone some time over the weekend. There's not any remaining by Monday morning.

Stocks

Quartz cuvettes

For our old spectrophotometer, we had two macrocell QS quartz cuvettes and two microcell QS quartz cuvettes. I tested the quality of these four cuvettes for the purposes of CD (circular dichroism) using the CD spectrometer at the Bioinstrumentation facility. The two microcell cuvettes were not suitable for CD ... they have high birefringence. The two macrocell cuvettes were suitable for CD in the wavelenth range 224-320nm. However, for protein structures like alpha helices, you'll likely want to check the spectra even at 200nm. So these cuvettes are not great for this purpose. A cuvette for use in CD was purchased.

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